BRENDA - Enzyme Database

Bakers yeast UMP:pyrophosphate phosphoribosyltransferase. Purification, enzymatic and kinetic properties

Natalini, P.; Ruggieri, S.; Santarelli, I.; Vita, A.; Magni, G.; J. Biol. Chem. 254, 1558-1563 (1979)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.4.2.9
enzyme is highly unstable, dimethyl sulfoxide stabilizes during purification
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.9
dCMP
allosteric inhibition
Saccharomyces cerevisiae
2.4.2.9
dCTP
allosteric inhibition
Saccharomyces cerevisiae
2.4.2.9
dUMP
allosteric inhibition
Saccharomyces cerevisiae
2.4.2.9
TTP
allosteric inhibition
Saccharomyces cerevisiae
2.4.2.9
UDP
allosteric inhibition
Saccharomyces cerevisiae
2.4.2.9
UMP
allosteric inhibition
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.9
0.021
-
Uracil
-
Saccharomyces cerevisiae
2.4.2.9
0.026
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.9
Mg2+
-
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.9
80000
-
gel filtration
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.9
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.9
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.9
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
638365
Saccharomyces cerevisiae
UMP + diphosphate
-
638365
Saccharomyces cerevisiae
?
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.4.2.9
22
42
-
Saccharomyces cerevisiae
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.4.2.9
60
-
2 min, complete loss of activity, 1 mM uracil + 0.1 EDTA protect partially
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.9
7.6
7.8
-
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.9
6.5
10
-
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.2.9
enzyme is highly unstable, dimethyl sulfoxide stabilizes during purification
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.9
dCMP
allosteric inhibition
Saccharomyces cerevisiae
2.4.2.9
dCTP
allosteric inhibition
Saccharomyces cerevisiae
2.4.2.9
dUMP
allosteric inhibition
Saccharomyces cerevisiae
2.4.2.9
TTP
allosteric inhibition
Saccharomyces cerevisiae
2.4.2.9
UDP
allosteric inhibition
Saccharomyces cerevisiae
2.4.2.9
UMP
allosteric inhibition
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.9
0.021
-
Uracil
-
Saccharomyces cerevisiae
2.4.2.9
0.026
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.9
Mg2+
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.9
80000
-
gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.9
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.9
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
638365
Saccharomyces cerevisiae
UMP + diphosphate
-
638365
Saccharomyces cerevisiae
?
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.4.2.9
22
42
-
Saccharomyces cerevisiae
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.4.2.9
60
-
2 min, complete loss of activity, 1 mM uracil + 0.1 EDTA protect partially
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.9
7.6
7.8
-
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.9
6.5
10
-
Saccharomyces cerevisiae