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Literature summary extracted from

  • Lussier, M.; Gentzsch, M.; Sdicu, A.M.; Bussey, H.; Tanner, W.
    Protein O-glycosylation in yeast. The PMT2 gene specifies a second protein O-mannosyltransferase that functions in addition to the PMT-1 encoded activity (1995), J. Biol. Chem., 270, 2770-2775.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.109 chromosome mapping of PMT1 Saccharomyces cerevisiae
2.4.1.109 DNA sequence determination, chromosome mapping Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.109 additional information PMT2 disruption mutant shows reduced in vitro and in vivo activity Saccharomyces cerevisiae
2.4.1.109 additional information PMT1 and PMT2 double disruption mutant shows severe growth defect but retain residual activity due to an additional enzyme Saccharomyces cerevisiae
2.4.1.109 additional information PMT2 haploid mutants grow slightly slower than the wild-type, the enzyme is required but not essential for normal vegetative growth of the cells Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.109 membrane integral, multiple transmembranal domains Saccharomyces cerevisiae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.109 Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.109 dolichyl phosphate D-mannose + protein Saccharomyces cerevisiae
-
 dolichyl phosphate + O-D-mannosylprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.109 Saccharomyces cerevisiae
-
 gene PMT1
-
2.4.1.109 Saccharomyces cerevisiae
-
 enzyme form other than PMT2
-
2.4.1.109 Saccharomyces cerevisiae P31382 PMT2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.109 glycoprotein PMT1 and PMT2 contain both 3 putative N-glycosylation sites Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.109 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.109 dolichyl phosphate D-mannose + Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
 Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
 ?
2.4.1.109 dolichyl phosphate D-mannose + protein
-
 Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
 ?
2.4.1.109 dolichyl phosphate D-mannose + protein the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.109 PMT
-
 Saccharomyces cerevisiae