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Literature summary extracted from

  • Narasimhan, S.
    Control of glycoprotein synthesis. UDP-GlcNAc:glycopeptide beta 4-N-acetylglucosaminyltransferase III, an enzyme in hen oviduct which adds GlcNAc in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-glycosyl oligosaccharides (1982), J. Biol. Chem., 257, 10235-10242.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.144 Triton X-100 activation, about 8fold at 0.13-1.3% v/v Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.144 0.23
-
 (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 Gallus gallus
2.4.1.144 1.1
-
 UDP-N-acetyl-D-glucosamine
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.144 membrane
-
 Gallus gallus 16020
-
2.4.1.144 microsome
-
 Gallus gallus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.144 Mn2+ broad optimum at 12 mM Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Gallus gallus R represents the remainder of the N-oligosaccharide core (+/-fucose), essential for biosynthesis of complex N-linked oligosaccharides UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.144 Gallus gallus
-
 hen, White Leghorn
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.144 oviduct
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.144 0.000083
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 Gallus gallus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R R represents the remainder of the N-oligosaccharide core (+/-fucose), essential for biosynthesis of complex N-linked oligosaccharides Gallus gallus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + GlcNAc-beta-1,2-Man-alpha-1,6-(GlcNAc-beta-1,2-Man-alpha-1,3)-Man-beta-1,4-GlcNAc-beta-1,4-(Fuc-alpha-1,6)-GlcNAc-Asn
-
 Gallus gallus UDP + GlcNAc-beta-1,2-Man-alpha-1,6-(GlcNAc-beta-1,2-Man-alpha-1,3)(GlcNAc-beta-1,4)-Man-beta-1,4-GlcNAc-beta-1,4-(Fuc-alpha-1,6)-GlcNAc-Asn incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine, both terminal beta-1,2-linked N-acetylglucosamine residues required for maximum activity, removal of one or both of them reduces activity by 85 or 93%, respectively ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.144 7
-
-
 Gallus gallus