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Literature summary extracted from
- 3-Phosphoshikimate 1-carboxyvinyltransferase from Escherichia coli (1987), Methods Enzymol., 142, 342-348.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.5.1.19 | Escherichia coli structural gene aroA | Escherichia coli |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.5.1.19 | dithiothreitol reverses aggregation which sometimes occurs after storage at -20°C | Escherichia coli |
| 2.5.1.19 | glycerol, 50% v/v, stabilizes | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.5.1.19 | N-phosphonomethylglycine | inhibition of enolpyruvate transfer, competitive vs. phosphoenolpyruvate | Escherichia coli |  |
| 2.5.1.19 | N-phosphonomethylglycine | - |
Klebsiella pneumoniae | |
| 2.5.1.19 | N-phosphonomethylglycine | - |
Neurospora crassa | |
| 2.5.1.19 | N-phosphonomethylglycine | - |
Pisum sativum | |
| 2.5.1.19 | N-phosphonomethylglycine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.5.1.19 | 0.00025 | - |
5-enolpyruvylshikimate 3-phosphate | - |
Neurospora crassa | |
| 2.5.1.19 | 0.00036 | - |
3-phosphoshikimate | - |
Neurospora crassa | |
| 2.5.1.19 | 0.0018 | - |
phosphate | - |
Neurospora crassa | |
| 2.5.1.19 | 0.0025 | - |
3-phosphoshikimate | recombinant overproducing strain | Escherichia coli | |
| 2.5.1.19 | 0.0027 | - |
phosphoenolpyruvate | - |
Neurospora crassa | |
| 2.5.1.19 | 0.003 | - |
5-enolpyruvylshikimate 3-phosphate | recombinant overproducing strain | Escherichia coli | |
| 2.5.1.19 | 0.0052 | - |
phosphoenolpyruvate | cosubstrate 5-enolpyruvylshikimate 3-phosphate | Pisum sativum | |
| 2.5.1.19 | 0.0077 | - |
3-phosphoshikimate | - |
Pisum sativum | |
| 2.5.1.19 | 0.016 | - |
phosphoenolpyruvate | recombinant overproducing strain | Escherichia coli | |
| 2.5.1.19 | 2.5 | - |
phosphate | - |
Escherichia coli | |
| 2.5.1.19 | 4 | - |
phosphate | - |
Pisum sativum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.5.1.19 | soluble | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
| 2.5.1.19 | soluble | - |
Escherichia coli | - |
- |
| 2.5.1.19 | soluble | - |
Neurospora crassa | - |
- |
| 2.5.1.19 | soluble | - |
Klebsiella pneumoniae | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.19 | 42000 | - |
gel filtration | Escherichia coli |
| 2.5.1.19 | 46110 | - |
calculated from polypeptide sequence of aroA gene product | Escherichia coli |
| 2.5.1.19 | 49000 | - |
1 * 49000 | Escherichia coli |
| 2.5.1.19 | 55000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | Neurospora crassa | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | Pisum sativum | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | Escherichia coli | sixth reaction of chorismate pathway | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.5.1.19 | Escherichia coli | - |
K-12 | - |
| 2.5.1.19 | Escherichia coli | - |
overproducing recombinant strain AB2829pKD501 | - |
| 2.5.1.19 | Klebsiella pneumoniae | - |
- |
- |
| 2.5.1.19 | Neurospora crassa | - |
- |
- |
| 2.5.1.19 | Pisum sativum | - |
- |
- |
| 2.5.1.19 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.5.1.19 | recombinant enzyme, ammonium sulfate, DEAE-Sephacel, phenyl-Sepharose, phosphocellulose chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.19 | 18 | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate, native enzyme | Escherichia coli |
| 2.5.1.19 | 62 | - |
phosphoenolpyruvate + 3-phosphoshikimate | Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.5.1.19 | -20°C, in 50% v/v glycerol, enzyme concentration 5 mg/ml, at least 1 year | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | - |
Neurospora crassa | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | - |
Pisum sativum | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | - |
Escherichia coli | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | - |
Neurospora crassa | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | - |
Pisum sativum | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | - |
Klebsiella pneumoniae | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
| 2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | sixth reaction of chorismate pathway | Escherichia coli | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.5.1.19 | monomer | - |
Pisum sativum |
| 2.5.1.19 | monomer | - |
Klebsiella pneumoniae |
| 2.5.1.19 | monomer | 1 * 49000 | Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.19 | 25 | - |
assay at | Escherichia coli |
| 2.5.1.19 | 25 | - |
assay at | Neurospora crassa |
| 2.5.1.19 | 25 | - |
assay at | Pisum sativum |
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