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Literature summary extracted from

  • Allen, M.; Qin, W.; Moreau, F.; Moffatt, B.
    Adenine phosphoribosyltransferase isoforms of Arabidopsis and their potential contributions to adenine and cytokinin metabolism (2002), Physiol. Plant., 115, 56-68.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.7 overexpression of the 3 isoforms in Escherichia coli Bl21(DE3)pLysS as His-tagged proteins, the majority of the recombiannt enzyme is in the soluble fraction Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.7 0.0008
-
 adenine APT3, pH 7.4 Arabidopsis thaliana
2.4.2.7 0.001
-
 adenine APT1, pH 7.4 Arabidopsis thaliana
2.4.2.7 0.0026
-
 adenine APT2, pH 7.4 Arabidopsis thaliana
2.4.2.7 0.015
-
 benzyladenine
-
 Arabidopsis thaliana
2.4.2.7 0.076
-
 zeatin
-
 Arabidopsis thaliana
2.4.2.7 0.11
-
 isopentenyladenine
-
 Arabidopsis thaliana
2.4.2.7 0.33
-
 zeatin
-
 Arabidopsis thaliana
2.4.2.7 0.44
-
 benzyladenine
-
 Arabidopsis thaliana
2.4.2.7 0.44
-
 isopentenyladenine
-
 Arabidopsis thaliana
2.4.2.7 1.8
-
 zeatin
-
 Arabidopsis thaliana
2.4.2.7 2.4
-
 benzyladenine
-
 Arabidopsis thaliana
2.4.2.7 2.5
-
 isopentenyladenine
-
 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.7 chloroplast
-
 Arabidopsis thaliana 9507
-
2.4.2.7 cytosol
-
 Arabidopsis thaliana 5829
-
2.4.2.7 nucleus
-
 Arabidopsis thaliana 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.7 Mg2+
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.7 28000
-
 x * 28000, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Arabidopsis thaliana P31166 isoform APT1
-
2.4.2.7 Arabidopsis thaliana Q42563 isoform APT2
-
2.4.2.7 Arabidopsis thaliana Q9LFP0 isoform APT5
-
2.4.2.7 Arabidopsis thaliana Q9SU38 isoform APT4
-
2.4.2.7 Arabidopsis thaliana Q9SUW2 isoform APT3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7 3 isoforms recombinant from Escherichia coli as His-tagged proteins Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.7 flower
-
 Arabidopsis thaliana
-
2.4.2.7 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate best acceptor substrate Arabidopsis thaliana AMP + diphosphate
-
 ?
2.4.2.7 benzyladenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Arabidopsis thaliana benzyladenosine 5'-phosphate + diphosphate
-
 ?
2.4.2.7 isopentenyladenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Arabidopsis thaliana isopentenyladenosine 5'-phosphate + diphosphate
-
 ?
2.4.2.7 zeatin + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Arabidopsis thaliana zeatin riboside 5'-phosphate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.7 ? x * 28000, SDS-PAGE Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.7 37
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.7 7.4 7.5 APT2 and 3, 37°C Arabidopsis thaliana
2.4.2.7 8.8
-
 APT1, 37°C Arabidopsis thaliana