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Literature summary extracted from

  • Martin, R.C.; Cloud, K.A.; Mok, M.C.; Mok, D.W.S.
    Substrate specificity and domain analyses of zeatin O-glycosyltransferases (2000), Plant Growth Regul., 32, 289-293.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.203
-
 Phaseolus lunatus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.203 additional information construction of chimeric recombinant enzyme mutants, exchange of the C-terminus with zeatin O-beta-D-xylosyltransferase, EC 2.4.2.40, gene ZOX1, determination of the site determining the substrate specificity Phaseolus lunatus
2.4.2.40 additional information construction of chimeric recombinant enzyme mutants, exchange of the C-terminus with zeatin O-beta-D-glucosyltransferase, EC 2.4.1.203, gene ZOG1, determination of the site determining the substrate specificity Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.40 UDP-glucose
-
 Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.203 Phaseolus lunatus
-
 gene ZOG1
-
2.4.1.203 Zea mays
-
-
-
2.4.2.40 Phaseolus vulgaris
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.203 UDP-glucose + trans-zeatin = UDP + O-beta-D-glucosyl-trans-zeatin localisation of site determining the substrate specificity for the UDP-sugar donors Phaseolus lunatus
a
2.4.2.40 UDP-D-xylose + zeatin = UDP + O-beta-D-xylosylzeatin localisation of site determining the substrate specificity for the UDP-sugar donors Phaseolus vulgaris
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.203 UDP-glucose + trans-zeatin UDP-glucose is the best donor substrate Phaseolus lunatus UDP + O-beta-D-glucosyl-trans-zeatin
-
 ?
2.4.1.203 UDP-xylose + dihydrozeatin converts dihydrozeatin exclusively with UDP-D-xylose as donor substrates Phaseolus lunatus UDP + O-beta-D-xylosyldihydrozeatin
-
 ?
2.4.2.40 UDP-D-xylose + dihydrozeatin
-
 Phaseolus vulgaris UDP + O-beta-D-xylosyldihydrozeatin
-
 ?
2.4.2.40 UDP-D-xylose + zeatin substrate specificity Phaseolus vulgaris UDP + O-beta-D-xylosylzeatin
-
 ?
2.4.2.40 UDP-D-xylose + zeatin absolutely specific for UDP-D-xylose Phaseolus vulgaris UDP + O-beta-D-xylosylzeatin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.203 8
-
 assay at Phaseolus lunatus
2.4.2.40 8
-
 assay at Phaseolus vulgaris