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Literature summary extracted from

  • Ober, D.; Harms, R.; Witte, L.; Hartmann, T.
    Molecular evolution by change of function. Alkaloid-specific homospermidine synthase retains all properties of deoxyhypusine synthase except binding the eIF5A precursor protein (2003), J. Biol. Chem., 278, 12805-12812.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.45 additional information
-
 additional information
-
 Senecio vernalis
2.5.1.46 additional information
-
 additional information
-
 Senecio vernalis
2.5.1.46 0.0013
-
 [eIF5A-precursor]-L-lysine
-
 Senecio vernalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.45 41000
-
 x * 41000, SDS-PAGE Senecio vernalis
2.5.1.46 41000
-
 x * 41000, SDS-PAGE Senecio vernalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.45 putrescine + spermine Senecio vernalis
-
 sym-homospermine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Senecio vernalis posttranslational activation of the initiation factor 5A [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.45 Senecio vernalis
-
-
-
2.5.1.46 Senecio vernalis Q9SC14
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.46 recombinant enzyme Senecio vernalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.45 putrescine + spermidine
-
 Senecio vernalis N-(4-aminobutyl)butane-1,4-diamine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermine
-
 Senecio vernalis sym-homospermine + propane-1,3-diamine
-
 ?
2.5.1.46 homospermidine + putrescine
-
 Senecio vernalis homospermidine + ?
-
 ?
2.5.1.46 spermidine + putrescine
-
 Senecio vernalis homospermidine + ?
-
 ?
2.5.1.46 [eIF5A-precursor]-L-lysine + spermidine
-
 Senecio vernalis [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Senecio vernalis [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine posttranslational activation of the initiation factor 5A Senecio vernalis [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.45 ? x * 41000, SDS-PAGE Senecio vernalis
2.5.1.46 ? x * 41000, SDS-PAGE Senecio vernalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.45 0.0032
-
 putrescine + spermidine Senecio vernalis
2.5.1.46 0.0022
-
 [eIF5A-precursor]-L-lysine + spermidine Senecio vernalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.45 9.3
-
-
 Senecio vernalis
2.5.1.46 9.3
-
 glycine-NaOH buffer Senecio vernalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.45 8.7 9.9 about 55% of maximal activity at pH 8.7 and at pH 9.9 Senecio vernalis
2.5.1.46 8.8 9.9 pH 8.8: about 35% of maximal activity, pH 9.9: about 60% of maximal activity Senecio vernalis