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Literature summary extracted from

  • Ober, D.; Hartmann, T.
    Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved form deoxyhypusine synthase (1999), Proc. Natl. Acad. Sci. USA, 96, 14777-14782.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.45
-
 Senecio vernalis
2.5.1.46
-
 Senecio vernalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.45 40700
-
 4 * 40700, calculation from sequence of cDNA Senecio vernalis
2.5.1.45 44500
-
 4 * 44500, SDS-PAGE Senecio vernalis
2.5.1.45 174000
-
 gel filtration Senecio vernalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.45 putrescine + spermine Senecio vernalis
-
 sym-homospermine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Senecio vernalis
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.45 Senecio vernalis Q9SC13
-
-
2.5.1.46 Senecio vernalis Q9SC14
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.45
-
 Senecio vernalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.45 root
-
 Senecio vernalis
-
2.5.1.45 tissue culture
-
 Senecio vernalis
-
2.5.1.46 shoot tip
-
 Senecio vernalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.46 0.0101
-
 with acceptor eIF5A-prcursor Senecio vernalis
2.5.1.46 0.0442
-
 with acceptor putrescine Senecio vernalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.45 putrescine + spermidine
-
 Senecio vernalis N-(4-aminobutyl)butane-1,4-diamine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermine
-
 Senecio vernalis sym-homospermine + propane-1,3-diamine
-
 ?
2.5.1.46 spermidine + putrescine
-
 Senecio vernalis homospermidine + ?
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Senecio vernalis [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Senecio vernalis [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine ?

Subunits

EC Number Subunits Comment Organism
2.5.1.45 tetramer 4 * 44500, SDS-PAGE Senecio vernalis
2.5.1.45 tetramer 4 * 40700, calculation from sequence of cDNA Senecio vernalis

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.45 NAD+
-
 Senecio vernalis
2.5.1.45 NADH
-
 Senecio vernalis
2.5.1.46 NAD+
-
 Senecio vernalis
2.5.1.46 NADH
-
 Senecio vernalis