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Literature summary extracted from

  • Mann, D.F.; Byerrum, R.U.
    Quinolinic acid phosphoribosyltransferase from castor bean endosperm. I. Purification and characterization (1974), J. Biol. Chem., 249, 6817-6823.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.19 2-Hydroxynicotinate 50% at 0.01 M Ricinus communis
2.4.2.19 methyl-3-amidopyridine-2-carboxylate
-
 Ricinus communis
2.4.2.19 methyl-3-cyanopyridine 2-carboxylate
-
 Ricinus communis
2.4.2.19 NAD+ 19% inhibition at 1 mM, 15% inhibition at 10 mM Ricinus communis
2.4.2.19 Picolinic acid
-
 Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.19 0.012
-
 quinolinate
-
 Ricinus communis
2.4.2.19 0.045
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.19 35000
-
 2 * 35000, SDS-PAGE Ricinus communis
2.4.2.19 68000
-
 gel filtration Ricinus communis
2.4.2.19 72000
-
 sucrose density gradient centrifugation Ricinus communis

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.19 Ricinus communis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.19 endosperm endosperm of etiolated seedlings Ricinus communis
-
2.4.2.19 seedling endosperm of etiolated seedlings Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.19 0.75
-
-
 Ricinus communis

Storage Stability

EC Number Storage Stability Organism
2.4.2.19 -90°C, stable for several months in 0.05 M potassium phosphate buffer, pH 7.0, 50% sucrose w/v and 0.01 M dithiothreitol Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.19 pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Ricinus communis nicotinate D-ribonucleotide + diphosphate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.19 dimer 2 * 35000, SDS-PAGE Ricinus communis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.19 additional information
-
 quinolinate at 0.8 mM gives 50% protection against heat inactivation. 22% inhibition in presence of quinolinate Ricinus communis
2.4.2.19 55
-
 10 min, 48% loss of activity Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.19 6.5 7.7
-
 Ricinus communis