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Literature summary extracted from

  • Sagami, H.; Ogura, K.
    Nonaprenylpyrophosphate synthetase from Micrococcus luteus (1985), Methods Enzymol., 110, 206-209.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.84 bacitracin 0.03-0.06%, 2fold stimulation Micrococcus luteus
2.5.1.84 Tween 80 0.05-0.1%, 2fold stimulation Micrococcus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.84 0.00025
-
 all-trans-farnesyl diphosphate pH 7.4, 37°C Micrococcus luteus
2.5.1.84 0.00075
-
 geranyl diphosphate pH 7.4, 37°C Micrococcus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.84 Mg2+ Mg2+ is absolutely required for activity, Mn2+ is less effective. The chain length of the synthesized polyprenyl diphosphate is dependent on the concentration of Mg2+ in the medium. At 0.1 mM Mg2+, C25, C30 and C35 compounds are produced with C25 predominating. At 0.5 mM Mg2+, C25, C30, C35 and C40 compounds are produced with C35 and c40 predominating. At 20 mM Mg2+, C40 and C45 are produced without accumulation od shorter compounds Micrococcus luteus
2.5.1.84 Mn2+ Mg2+ is absolutely required for activity, Mn2+ is less effective Micrococcus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.84 78000
-
 gel filtration Micrococcus luteus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.84 Micrococcus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.84
-
 Micrococcus luteus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.84 0.000787
-
-
 Micrococcus luteus

Storage Stability

EC Number Storage Stability Organism
2.5.1.84 -20°C, stable for at least 2 weeks Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.84 all-trans-farnesyl diphosphate + 6 isopentenyl diphosphate
-
 Micrococcus luteus 6 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?
2.5.1.84 geranyl diphosphate + 7 isopentenyl diphosphate
-
 Micrococcus luteus 7 diphosphate + all-trans-nonaprenyl diphosphate the chain length of the synthesized polyprenyl diphosphate is dependent on the concentration of Mg2+ in the medium. At 0.1 mM Mg2+, C25, C30 and C35 compounds are produced with C25 predominating. At 0.5 mM Mg2+, C25, C30, C35 and C40 compounds are produced with C35 and C40 predominating. At 20 mM Mg2+, C40 and C45 are produced without accumulation od shorter compounds ?
2.5.1.84 geranylgeranyl diphosphate + 5 isopentenyl diphosphate
-
 Micrococcus luteus 5 diphosphate + all-trans-nonaprenyl diphosphate
-
 ?
2.5.1.84 additional information no activity with geranylneryl diphosphate or dimethylallyl diphosphate Micrococcus luteus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.84 nonaprenylpyrophosphate synthetase
-
 Micrococcus luteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.84 37
-
 assay at Micrococcus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.84 7 8 in Tris-HCl buffer Micrococcus luteus