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Literature summary extracted from

  • Reddy, C.C.; Burgess, J.R.; Tu, C.P.D.
    Isolation and characterization of an anionic glutathione S-transferase from rat liver cytosol (1983), Biochem. Biophys. Res. Commun., 111, 840-846.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.18 loss of activity during purification due to irreversible inactivation Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.23
-
 glutathione
-
 Rattus norvegicus
2.5.1.18 0.94
-
 1-chloro-2,4-dinitrobenzene
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 26000
-
 2 * 26000, SDS-PAGE Rattus norvegicus
2.5.1.18 49000 54000 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 homogeneity, anionic enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 10.83
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-dichloro-4-nitrobenzene
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Rattus norvegicus S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 4-nitrobenzyl chloride
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + 4-nitrophenyl acetate
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + ethacrynic acid
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 additional information specificity Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 2 * 26000, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 7.33
-
 glutathione
-
 Rattus norvegicus