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Literature summary extracted from

  • Asaoka, K.; Takahashi, K.
    Purification and properties of porcine brain glutathione S-transferases (1983), J. Biochem., 94, 1191-1199.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.18 loss of activity during dialysis Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 cytosol
-
 Sus scrofa 5829
-
2.5.1.18 microsome
-
 Sus scrofa
-
-
2.5.1.18 mitochondrion
-
 Sus scrofa 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 22000
-
 2 * 22000, SDS-PAGE Sus scrofa
2.5.1.18 43000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 homogeneity, 4 enzyme fractions Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 brain distribution in brain Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 124
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-dinitrobenzene
-
 Sus scrofa ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Sus scrofa S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 2,5-dinitrophenol
-
 Sus scrofa ?
-
 ?
2.5.1.18 glutathione + 3,4-dinitrobenzoic acid
-
 Sus scrofa ?
-
 ?
2.5.1.18 glutathione + 4-nitrobenzyl chloride
-
 Sus scrofa ?
-
 ?
2.5.1.18 glutathione + 5-nitrofurfural
-
 Sus scrofa ?
-
 ?
2.5.1.18 glutathione + 5-nitrofurfural diacetal
-
 Sus scrofa ?
-
 ?
2.5.1.18 additional information specificity Sus scrofa ?
-
 ?
2.5.1.18 RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Sus scrofa HX + R-S-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 2 * 22000, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 6.5 7.5
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 6 8.1 at pH 6.0 and 8.1 about 50% of activity maximum Sus scrofa