Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Alin, P.; Jensson, H.; Guthenberg, C.; Danielson, U.H.; Tahir, M.K.; Mannervik, B.
    Purification of major basic glutathione transferase isoenzymes from rat liver by use of affinity chromatography and fast protein liquid chromatofocusing (1985), Anal. Biochem., 146, 313-320.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 bromosulfophthalein
-
 Rattus norvegicus
2.5.1.18 Cibacron blue
-
 Rattus norvegicus
2.5.1.18 Hematin
-
 Rattus norvegicus
2.5.1.18 indomethacin
-
 Rattus norvegicus
2.5.1.18 Rose bengal
-
 Rattus norvegicus
2.5.1.18 S-(4-Bromobenzyl)glutathione
-
 Rattus norvegicus
2.5.1.18 sulfophthalein
-
 Rattus norvegicus
2.5.1.18 Tributyltin acetate
-
 Rattus norvegicus
2.5.1.18 Triethyltin bromide
-
 Rattus norvegicus
2.5.1.18 Triphenyltin chloride
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 additional information
-
 additional information
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 cytosol
-
 Rattus norvegicus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.18 RX + glutathione Rattus norvegicus detoxification of products of oxidative metabolism, e.g. 4-hydroxyalkenals, epoxides, organic hydroperoxides HX + R-S-glutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Rattus norvegicus
-
 male specific-pathogen-free Sprague Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 7 major isoenzymes Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 16.7 64.1 with 1-chloro-2,4-dinitrobenzene as substrate Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-dichloro-4-nitrobenzene
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Rattus norvegicus S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 4-nitrophenyl acetate
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + androst-5-ene-3,17-dione
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + ethacrynic acid
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + trans-4-phenyl-3-buten-2-one
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 additional information specificity Rattus norvegicus ?
-
 ?
2.5.1.18 RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Rattus norvegicus HX + R-S-glutathione
-
 ?
2.5.1.18 RX + glutathione detoxification of products of oxidative metabolism, e.g. 4-hydroxyalkenals, epoxides, organic hydroperoxides Rattus norvegicus HX + R-S-glutathione
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 30
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 27
-
 1-chloro-2,4-dinitrobenzene isoenzyme 4-4, cofactor: glutathione Rattus norvegicus
2.5.1.18 54
-
 1-chloro-2,4-dinitrobenzene isoenzyme 3-3, cofactor: glutathione Rattus norvegicus
2.5.1.18 63
-
 1-chloro-2,4-dinitrobenzene isoenzyme 1-2, cofactor: glutathione Rattus norvegicus
2.5.1.18 65
-
 1-chloro-2,4-dinitrobenzene isoenzyme 3-4, cofactor: glutathione Rattus norvegicus
2.5.1.18 78
-
 1-chloro-2,4-dinitrobenzene isoenzyme 1-1, cofactor: glutathione Rattus norvegicus
2.5.1.18 82
-
 1-chloro-2,4-dinitrobenzene isoenzyme 3-?, cofactor: glutathione Rattus norvegicus