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Literature summary extracted from

  • Yeung, T.C.; Gidari, A.S.
    Purification and properties of a chicken liver glutathione S-transferase (1980), Arch. Biochem. Biophys., 205, 404-411.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 hemin competitive with 1-chloro-2,4-dinitrobenzene, non-competitive with glutathione Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.35
-
 reduced glutathione
-
 Gallus gallus
2.5.1.18 1.92
-
 1-chloro-2,4-dinitrobenzene
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 cytosol
-
 Gallus gallus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 23000
-
 2 * 23000, SDS-PAGE Gallus gallus
2.5.1.18 45000
-
 gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 liver foetal Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 0.03
-
 with 1-chloro-2,4-dinitrobenzene + glutathione or trans-4-phenyl-3-buten-2-one as substrate Gallus gallus
2.5.1.18 0.49
-
 with 1,2-epoxy-3-(4-nitrophenoxy)propane as substrate Gallus gallus
2.5.1.18 0.75
-
 with 4-nitrobenzyl chloride as substrate Gallus gallus
2.5.1.18 0.83
-
 with ethacrynic acid as substrate Gallus gallus
2.5.1.18 3.44
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-dichloro-4-nitrobenzene
-
 Gallus gallus ?
-
 ?
2.5.1.18 glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane
-
 Gallus gallus ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Gallus gallus S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 4-nitrobenzyl chloride
-
 Gallus gallus ?
-
 ?
2.5.1.18 glutathione + ethacrynic acid
-
 Gallus gallus ?
-
 ?
2.5.1.18 glutathione + trans-4-phenyl-3-buten-2-one
-
 Gallus gallus ?
-
 ?
2.5.1.18 additional information obligate requirement for reduced glutathione as thiol donor Gallus gallus ?
-
 ?
2.5.1.18 RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Gallus gallus HX + R-S-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 2 * 23000, SDS-PAGE Gallus gallus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 45
-
 10 min, 50% loss of activity Gallus gallus
2.5.1.18 80
-
 complete inactivation after 10 min Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 8.8
-
-
 Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.18 0.001
-
 hemin with reduced glutathione Gallus gallus