Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schledz, M.; Al-Babili, S.; Lintig, J.v.; Haubruck, H.; Rabbani, S.; Kleinig, H.; Beyer, P.
    Phytoene synthase from Narcissus pseudonarcissus: functional expression, galactolipid requirement, topological distribution in chromoplasts and induction during flowering (1996), Plant J., 10, 781-792.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.32 galactose or galactose moieties in galactolipids are required Narcissus pseudonarcissus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.32 overexpressed in insect Sf9 cells using the baculovirus lipofection system. The full-length overexpressed enzyme exhibits very reduced catalytic activity compared with an overexpressed N-truncated form, with its transit sequence of 214 base pairs removed from the psy cDNA at the 5'-end by site-directed mutagenesis. The shortened form readily binds to lipid bilayers Narcissus pseudonarcissus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.32 additional information the full-length overexpressed enzyme exhibits very reduced catalytic activity compared with an overexpressed N-truncated form, with its transit sequence of 214 base pairs removed from the psy cDNA at the 5'-end by site-directed mutagenesis. The shortened form readily binds to lipid bilayers Narcissus pseudonarcissus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.32 fructose
-
 Narcissus pseudonarcissus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.32 chromoplast
-
 Narcissus pseudonarcissus 9509
-
2.5.1.32 membrane the soluble enzyme is complexed and inactive, the membrane-bound enzyme is active Narcissus pseudonarcissus 16020
-
2.5.1.32 soluble the soluble enzyme is complexed and inactive, the membrane-bound enzyme is active Narcissus pseudonarcissus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.32 Mg2+ stimulatory in presence of Mn2+ Narcissus pseudonarcissus
2.5.1.32 Mn2+ absolute requirement, optimum at 1 mM Narcissus pseudonarcissus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.32 Narcissus pseudonarcissus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.32
-
 Narcissus pseudonarcissus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.32 flower inner corona of, in all stages the steady-state phytoene synthase transcript levels are low Narcissus pseudonarcissus
-
2.5.1.32 additional information more activity in green leaves Narcissus pseudonarcissus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.32 geranylgeranyl diphosphate + geranylgeranyl diphosphate
-
 Narcissus pseudonarcissus prephytoene diphosphate + diphosphate
-
 ?