EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.32 | ATP | required | Pantoea ananatis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.32 | overexpressed to about 20% of the total cellular protein in Escherichia coli. Formation of the active phytoene synthase has the effect of suppressing the growth of the expressing strain. Inhibition of growth arises from the depletion of the substrate geranylgeranyl diphosphate which, in Escherichia coli, is necesssary for the synthesis of essential propyldiphosphate derivatives. In order to overcome the poor growth characteristics of the phytoene expressing strain, the enzyme level is increased by co-expressing the isoprenoid biosynthetic genes crtE and idi, encoding Erwinia geranylgeranyl diphosphate synthase and Rhodobacter isopentenyl diphosphate isomerase, respectively | Pantoea ananatis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.32 | phosphate | - |
Pantoea ananatis | |
2.5.1.32 | squalestatin | IC50: 0.015 mM | Pantoea ananatis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.32 | Mg2+ | Mn2+ or Mg2+ required | Pantoea ananatis | |
2.5.1.32 | Mn2+ | Mn2+ or Mg2+ required | Pantoea ananatis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.32 | Pantoea ananatis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.32 | - |
Pantoea ananatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.32 | geranylgeranyl diphosphate + geranylgeranyl diphosphate | the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene | Pantoea ananatis | prephytoene diphosphate + diphosphate | - |
? | |
2.5.1.32 | prephytoene diphosphate | the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene | Pantoea ananatis | phytoene + diphosphate | - |
? | |
2.5.1.32 | prephytoene diphosphate | the phytoene formed in vitro is present in both a 15-cis and all-trans isomeric configuration | Pantoea ananatis | phytoene + diphosphate | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.32 | ATP | required | Pantoea ananatis |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.5.1.32 | 0.015 | - |
IC50: 0.015 mM | Pantoea ananatis | squalestatin |