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Literature summary extracted from

  • Tanaka, K.; Kawaguchi, T.; Imada, Y.; Ooi, T.; Arai, M.
    Purification and properties of cellobiose phosphorylase from Clostridium thermocellum (1995), J. Ferment. Bioeng., 79, 212-216.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.20 dithiothreitol
-
Acetivibrio thermocellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.20 D-glucose strong inhibition at high concentrations Acetivibrio thermocellus
2.4.1.20 N-ethylmaleimide
-
Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.20 8
-
alpha-D-glucose 1-phosphate
-
Acetivibrio thermocellus
2.4.1.20 10
-
D-glucosamine
-
Acetivibrio thermocellus
2.4.1.20 40
-
D-xylose
-
Acetivibrio thermocellus
2.4.1.20 170
-
D-mannose
-
Acetivibrio thermocellus
2.4.1.20 250
-
2-deoxy-D-glucose
-
Acetivibrio thermocellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.20 Mg2+ not required Acetivibrio thermocellus
2.4.1.20 additional information no influence of metal ions on reaction Acetivibrio thermocellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.20 85000
-
SDS-PAGE Acetivibrio thermocellus
2.4.1.20 150000
-
gel filtration Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.20 cellobiose + phosphate Acetivibrio thermocellus
-
alpha-D-glucose 1-phosphate + D-glucose
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.20 Acetivibrio thermocellus
-
strain ATCC 27405
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.20
-
Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.20 20
-
purified enzyme Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.20 alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
Acetivibrio thermocellus ?
-
?
2.4.1.20 alpha-D-glucose 1-phosphate + D-arabinose very poor substrate Acetivibrio thermocellus ?
-
?
2.4.1.20 alpha-D-glucose 1-phosphate + D-glucosamine
-
Acetivibrio thermocellus ?
-
?
2.4.1.20 alpha-D-glucose 1-phosphate + D-mannose
-
Acetivibrio thermocellus ?
-
?
2.4.1.20 alpha-D-glucose 1-phosphate + D-xylose
-
Acetivibrio thermocellus ?
-
?
2.4.1.20 cellobiose + phosphate
-
Acetivibrio thermocellus alpha-D-glucose 1-phosphate + D-glucose
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.20 dimer 2 * 85000, gel filtration, SDS-PAGE Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.20 60
-
-
Acetivibrio thermocellus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.20 20 80 very low activity at 20 and 80°C Acetivibrio thermocellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.20 50
-
unstable above Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.20 6 9
-
Acetivibrio thermocellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.20 3 10
-
Acetivibrio thermocellus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.20 4 9 most stable at pH 7, rapid loss of activity at different pH Acetivibrio thermocellus