EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.20 | dithiothreitol | - |
Acetivibrio thermocellus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.20 | D-glucose | strong inhibition at high concentrations | Acetivibrio thermocellus | |
2.4.1.20 | N-ethylmaleimide | - |
Acetivibrio thermocellus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.20 | 8 | - |
alpha-D-glucose 1-phosphate | - |
Acetivibrio thermocellus | |
2.4.1.20 | 10 | - |
D-glucosamine | - |
Acetivibrio thermocellus | |
2.4.1.20 | 40 | - |
D-xylose | - |
Acetivibrio thermocellus | |
2.4.1.20 | 170 | - |
D-mannose | - |
Acetivibrio thermocellus | |
2.4.1.20 | 250 | - |
2-deoxy-D-glucose | - |
Acetivibrio thermocellus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.20 | Mg2+ | not required | Acetivibrio thermocellus | |
2.4.1.20 | additional information | no influence of metal ions on reaction | Acetivibrio thermocellus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 85000 | - |
SDS-PAGE | Acetivibrio thermocellus |
2.4.1.20 | 150000 | - |
gel filtration | Acetivibrio thermocellus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.20 | cellobiose + phosphate | Acetivibrio thermocellus | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.20 | Acetivibrio thermocellus | - |
strain ATCC 27405 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.20 | - |
Acetivibrio thermocellus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 20 | - |
purified enzyme | Acetivibrio thermocellus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.20 | alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Acetivibrio thermocellus | ? | - |
? | |
2.4.1.20 | alpha-D-glucose 1-phosphate + D-arabinose | very poor substrate | Acetivibrio thermocellus | ? | - |
? | |
2.4.1.20 | alpha-D-glucose 1-phosphate + D-glucosamine | - |
Acetivibrio thermocellus | ? | - |
? | |
2.4.1.20 | alpha-D-glucose 1-phosphate + D-mannose | - |
Acetivibrio thermocellus | ? | - |
? | |
2.4.1.20 | alpha-D-glucose 1-phosphate + D-xylose | - |
Acetivibrio thermocellus | ? | - |
? | |
2.4.1.20 | cellobiose + phosphate | - |
Acetivibrio thermocellus | alpha-D-glucose 1-phosphate + D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.20 | dimer | 2 * 85000, gel filtration, SDS-PAGE | Acetivibrio thermocellus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 60 | - |
- |
Acetivibrio thermocellus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 20 | 80 | very low activity at 20 and 80°C | Acetivibrio thermocellus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 50 | - |
unstable above | Acetivibrio thermocellus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 6 | 9 | - |
Acetivibrio thermocellus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 3 | 10 | - |
Acetivibrio thermocellus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 4 | 9 | most stable at pH 7, rapid loss of activity at different pH | Acetivibrio thermocellus |