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Literature summary extracted from

  • Yamagata, S.
    Homocysteine in yeast. Partial purification and properties of O-acetylhomoserine sulfhydrylase (1971), J. Biochem., 70, 1035-1045.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.49 L-homoserine competitive inhibition Saccharomyces cerevisiae
2.5.1.49 L-methionine competitive inhibition Saccharomyces cerevisiae
2.5.1.49 additional information L-serine, glycine and L-alanine are not inhibitors, dithiothreitol and p-chloromercuribenzoate shows no inhibitory effect Saccharomyces cerevisiae
2.5.1.49 O-acetyl-L-serine competitive inhibition Saccharomyces cerevisiae
2.5.1.49 O-succinyl-DL-homoserine competitive inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.49 4.55
-
 O-acetyl-L-homoserine
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.49 480000
-
 gel filtration, larger than, no correct value could be estimated Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.49 O-acetyl-L-homoserine + H2S Saccharomyces cerevisiae
-
 L-homocysteine + acetic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.49 Saccharomyces cerevisiae
-
 baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.49 partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.49 19.2
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.49 additional information O-acetyl-L-threonine, O-acetyl-L-tyrosine, O-acetyl-L-hydroxyproline and O-succinyl-DL-homoserine are no substrates Saccharomyces cerevisiae ?
-
 ?
2.5.1.49 additional information cystathionine is not synthesized from O-acetylhomoserine and cysteine, O-acetyl-L-serine is no substrate Saccharomyces cerevisiae ?
-
 ?
2.5.1.49 O-acetyl-L-homoserine + ethyl mercaptan
-
 Saccharomyces cerevisiae L-ethionine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + H2S
-
 Saccharomyces cerevisiae L-homocysteine + acetic acid
-
 ?
2.5.1.49 O-acetyl-L-homoserine + methyl mercaptan
-
 Saccharomyces cerevisiae L-methionine + acetic acid
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.49 7.8
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.49 5.5 8.5
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.49 7.8 8 most stable at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.49 pyridoxal 5'-phosphate absolutely required for activity, Km 0.00294 mM Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.49 0.9
-
 L-methionine
-
 Saccharomyces cerevisiae
2.5.1.49 16
-
 L-homoserine
-
 Saccharomyces cerevisiae
2.5.1.49 19
-
 O-succinyl-DL-homoserine
-
 Saccharomyces cerevisiae
2.5.1.49 21.8
-
 O-acetyl-L-serine
-
 Saccharomyces cerevisiae