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Literature summary extracted from

  • Chen, H.; Blume, A.; Zimmermann-Kordmann, M.; Reutter, W.; Hinderlich, S.
    Purification and characterization of N-acetylneuraminic acid-9-phosphate synthase from rat liver (2002), Glycobiology, 12, 65-71.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.57
-
 Homo sapiens

General Stability

EC Number General Stability Organism
2.5.1.57 protected from inactivation by presence of phosphoenolpyruvate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.57 5,5'-dithio-bis(2-nitrobenzoic acid)
-
 Rattus norvegicus
2.5.1.57 N-ethylmaleimide
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.57 0.035
-
 N-acetyl-D-mannosamine 6-phosphate
-
 Rattus norvegicus
2.5.1.57 0.1
-
 phosphoenolpyruvate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.57 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.57 Fe2+ absolute requirement for divalent cations Rattus norvegicus
2.5.1.57 Mg2+ absolute requirement for divalent cations Rattus norvegicus
2.5.1.57 Mn2+ absolute requirement for divalent cations Rattus norvegicus
2.5.1.57 additional information Ca2+ or Cu2+ does not substitute for Mg2+ Rattus norvegicus
2.5.1.57 Ni2+ absolute requirement for divalent cations Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.57 37000
-
 2 * 37000, SDS-PAGE Rattus norvegicus
2.5.1.57 75000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O Escherichia coli
-
 N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O Rattus norvegicus
-
 N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.57 Escherichia coli
-
 neuB gene product amino acid sequence accession. no.
-
2.5.1.57 Homo sapiens Q9NR45 amino acid sequence
-
2.5.1.57 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.57
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.57 liver
-
 Rattus norvegicus
-
2.5.1.57 salivary gland
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.57 0.505
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.5.1.57 4°C, crude extract retains 60% of enzyme activity when stored for 1 week Rattus norvegicus
2.5.1.57 4°C, purified enzyme loses activity completely after 1 day in phosphate buffer Rattus norvegicus
2.5.1.57 4°C, purified enzyme, 50% of enzyme activity remains after 2 days in phosphate buffer containing 5 mM MgCl2, enzyme activity is lost entirely after 3 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Escherichia coli N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?
2.5.1.57 N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Rattus norvegicus N-acetyl-neuraminate 9-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.57 dimer 2 * 37000, SDS-PAGE Rattus norvegicus