BRENDA - Enzyme Database

UDPG:sterol glucosyltransferase in etiolated pea seedlings

Fang, T.Y.; Baisted, D.J.; Phytochemistry 15, 273-278 (1976)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.173
ATP
slightly stimulation at low concentration
Pisum sativum
2.4.1.173
phosphatidylcholine
33% increase of activity
Pisum sativum
2.4.1.173
phosphatidylethanolamine
increases 125% of activity
Pisum sativum
2.4.1.173
phosphatidylserine
33% increase of activity
Pisum sativum
2.4.1.173
phospholipase
treatment with phospholipases A, C and D lowers activity 21-29%, the effect of phospholipase A is completely reverse by phosphatidylethanolamine but not by phosphatidylcholine or phosphatidylserine, after phospholipase C or D treatment each phospholipid brings about a partial recovery of activity but phosphatidylethanolamine is superior
Pisum sativum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.173
ATP
inhibition at 1-3 mM but slightly stimulation at low concentration
Pisum sativum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.173
etioplast
-
Pisum sativum
9513
-
2.4.1.173
Golgi apparatus
-
Pisum sativum
5794
-
2.4.1.173
mitochondrion
-
Pisum sativum
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.173
Ca2+
maximal stimulation at 13 mM
Pisum sativum
2.4.1.173
additional information
no metal ion requirement
Pisum sativum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.173
Pisum sativum
-
pea
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.173
partial purification by differential centrifugation
Pisum sativum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.173
seedling
etiolated
Pisum sativum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.173
UDP-glucose + sitosterol
-
637775
Pisum sativum
UDP + sitosterol 3-beta-D-glucoside
-
637775
Pisum sativum
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.173
30
-
assay at
Pisum sativum
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.1.173
8
-
Tris-HCl 25 mM
Pisum sativum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.173
ATP
slightly stimulation at low concentration
Pisum sativum
2.4.1.173
phosphatidylcholine
33% increase of activity
Pisum sativum
2.4.1.173
phosphatidylethanolamine
increases 125% of activity
Pisum sativum
2.4.1.173
phosphatidylserine
33% increase of activity
Pisum sativum
2.4.1.173
phospholipase
treatment with phospholipases A, C and D lowers activity 21-29%, the effect of phospholipase A is completely reverse by phosphatidylethanolamine but not by phosphatidylcholine or phosphatidylserine, after phospholipase C or D treatment each phospholipid brings about a partial recovery of activity but phosphatidylethanolamine is superior
Pisum sativum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.173
ATP
inhibition at 1-3 mM but slightly stimulation at low concentration
Pisum sativum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.173
etioplast
-
Pisum sativum
9513
-
2.4.1.173
Golgi apparatus
-
Pisum sativum
5794
-
2.4.1.173
mitochondrion
-
Pisum sativum
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.173
Ca2+
maximal stimulation at 13 mM
Pisum sativum
2.4.1.173
additional information
no metal ion requirement
Pisum sativum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.173
partial purification by differential centrifugation
Pisum sativum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.173
seedling
etiolated
Pisum sativum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.173
UDP-glucose + sitosterol
-
637775
Pisum sativum
UDP + sitosterol 3-beta-D-glucoside
-
637775
Pisum sativum
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.173
30
-
assay at
Pisum sativum
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.1.173
8
-
Tris-HCl 25 mM
Pisum sativum