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Literature summary extracted from

  • Hayakawa, T.; Shibata, K.; Iwai, K.
    Purification and some properties of nicotinate phosphoribosyltransferase from hog liver (1984), Agric. Biol. Chem., 48, 445-453.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.21 additional information no activation by ADP Sus scrofa
6.3.4.21 additional information no activation by AMP, 2'-AMP Sus scrofa
6.3.4.21 additional information no activation by 3'-AMP, 5'-AMP Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.21 ATP in the presence of Mn2+ Rattus norvegicus
6.3.4.21 ATP in the presence of Mn2+ Sus scrofa
6.3.4.21 GDP
-
 Sus scrofa
6.3.4.21 GMP
-
 Sus scrofa
6.3.4.21 IDP
-
 Sus scrofa
6.3.4.21 IMP
-
 Sus scrofa
6.3.4.21 ITP
-
 Sus scrofa
6.3.4.21 Monoiodoacetic acid 1 mM and above Sus scrofa
6.3.4.21 additional information no inhibition by antibodies against pig kidney quinolinate phosphoribosyltransferase; no inhibition by nicotinamide mononucleotide, deamino-NAD+ Sus scrofa
6.3.4.21 N-ethylmaleimide 0.1 mM and above Sus scrofa
6.3.4.21 NAD+ weak inhibitor Sus scrofa
6.3.4.21 NADH weak inhibitor Sus scrofa
6.3.4.21 NADP+ weak inhibitor Sus scrofa
6.3.4.21 NADPH weak inhibitor Sus scrofa
6.3.4.21 Nicotinate analogues not Sus scrofa
6.3.4.21 nicotinate mononucleotide competitive to nicotinate and noncompetitive to phosphoribobose diphosphate Sus scrofa
6.3.4.21 PCMB 0.01 mM and above Sus scrofa
6.3.4.21 Pyrazine 2-carboxylic acid
-
 Sus scrofa
6.3.4.21 Pyridazine-4,5-dicarboxylic acid weak, 10% less activity Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.21 Co2+ requirement, slight synergism with ATP, more effective than Mg2+ with or without ATP Sus scrofa
6.3.4.21 Fe2+ slight stimulation, synergism with ATP Sus scrofa
6.3.4.21 Mg2+ requirement Sus scrofa
6.3.4.21 Mg2+ synergism with ATP Sus scrofa
6.3.4.21 Mn2+ requirement, 7.5 mM, no synergism with ATP, more effective than Mg2+ with or without ATP Sus scrofa
6.3.4.21 additional information absolute requirement of divalent cation, no activation with Ba2+, Ca2+, Cu2+, Cd2+, Al3+, Fe3+ Sus scrofa
6.3.4.21 Zn2+ slight stimulation Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.21 63000
-
 2 * 63000, SDS-PAGE Sus scrofa
6.3.4.21 120000
-
 liver, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.21 Rattus norvegicus
-
 male Wistar strain
-
6.3.4.21 Rattus norvegicus male Wistar
-
 male Wistar strain
-
6.3.4.21 Sus scrofa
-
 hog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.21 ammonium hydroxide precipitation, chromatography on DEAE-Sephadex, Sephacryl and phenyl-Sepharose Sus scrofa
6.3.4.21 homogenates Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.21 blood clot
-
 Rattus norvegicus
-
6.3.4.21 heart
-
 Rattus norvegicus
-
6.3.4.21 kidney
-
 Rattus norvegicus
-
6.3.4.21 liver
-
 Rattus norvegicus
-
6.3.4.21 liver
-
 Sus scrofa
-
6.3.4.21 additional information distribution in rat tissues: trace activities in brain, lung or stomach, not in thigh muscle, testis or serum Rattus norvegicus
-
6.3.4.21 pancreas
-
 Rattus norvegicus
-
6.3.4.21 small intestine
-
 Rattus norvegicus
-
6.3.4.21 spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.21 0.095
-
 crude extract Sus scrofa
6.3.4.21 160.5
-
 DEAE-Sephadex fraction Sus scrofa
6.3.4.21 421.2
-
 Sephacryl fraction Sus scrofa
6.3.4.21 457.3
-
 phenyl-Sepharose fraction Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
 Rattus norvegicus beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O no phosphoribosylation of nicotinamide, quinolic acid, adenine or hypoxanthine Sus scrofa beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
 Rattus norvegicus male Wistar beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.21 dimer 2 * 63000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
6.3.4.21 NPRTase
-
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.21 37
-
 assay at Rattus norvegicus
6.3.4.21 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.21 additional information
-
 pI: 4.8 Sus scrofa
6.3.4.21 7.3 7.4
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.21 ATP activation Rattus norvegicus
6.3.4.21 ATP activation Sus scrofa
6.3.4.21 ATP together with Mg2+ Sus scrofa
6.3.4.21 ATP together with Co2+ Sus scrofa
6.3.4.21 GTP not Sus scrofa
6.3.4.21 ITP not Sus scrofa