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Literature summary extracted from

  • Hanna, L.S.; Hess, S.L.; Sloan, D.L.
    Kinetic analysis of nicotinate phosphoribosyltransferase from yeast using high pressure liquid chromatography (1983), J. Biol. Chem., 258, 9745-9754.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.21 5-phospho-alpha-D-ribose 1-diphosphate at high concentrations Saccharomyces cerevisiae
6.3.4.21 nicotinate mononucleotide
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.21 0.023
-
 nicotinate
-
 Saccharomyces cerevisiae
6.3.4.21 0.024
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharomyces cerevisiae
6.3.4.21 0.07
-
 ATP
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.21 45000
-
 SDS-PAGE in the presence and absence of mercaptoethanol Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O Saccharomyces cerevisiae
-
 beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.21 Saccharomyces cerevisiae
-
 Budweiser brand
-
6.3.4.21 Saccharomyces cerevisiae
-
 bakers´yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.21 extraction, fractionation chromatography on phosphocellulose, hydroxyapatite, blue Sephadex and DEAE-cellulose Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate ordered ping pong kinetic mechanism Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.21 additional information ATP and phosphoribose diphosphate compete for ATP-binding site Saccharomyces cerevisiae ?
-
 ?
6.3.4.21 additional information ATPase activity in the presence of either product and in the absence of phosphoribose diphosphate Saccharomyces cerevisiae ?
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
 Saccharomyces cerevisiae beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.21 additional information
-
 pI: 6.9 Saccharomyces cerevisiae