Literature summary extracted from
Kosaka, A.; Spivey, H.O.; Gholson, R.K.
Nicotinate phosphoribosyltransferase of yeast. Purification and properties (1971), J. Biol. Chem., 246, 3277-3283.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
6.3.4.21 |
additional information |
no activation by ADP |
Saccharomyces cerevisiae |
|
6.3.4.21 |
additional information |
no activation by 3'-AMP, 5'-AMP |
Saccharomyces cerevisiae |
|
General Stability
EC Number |
General Stability |
Organism |
---|
6.3.4.21 |
heat, unstable to, substrates protect |
Saccharomyces cerevisiae |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
6.3.4.21 |
Mg2+ |
above 10 mM, significant pH alterations |
Saccharomyces cerevisiae |
|
6.3.4.21 |
phosphate |
0.1 M and above |
Saccharomyces cerevisiae |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
6.3.4.21 |
0.09 |
- |
ATP |
- |
Saccharomyces cerevisiae |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
6.3.4.21 |
Mg2+ |
requirement |
Saccharomyces cerevisiae |
|
6.3.4.21 |
Mg2+ |
synergism with ATP |
Saccharomyces cerevisiae |
|
6.3.4.21 |
Mg2+ |
1-10 mM |
Saccharomyces cerevisiae |
|
6.3.4.21 |
MgATP2- |
- |
Saccharomyces cerevisiae |
|
6.3.4.21 |
phosphate |
activation, 0.01-0.1 M, in the presence of Mg2+ |
Saccharomyces cerevisiae |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
6.3.4.21 |
43000 |
- |
gel filtration |
Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
6.3.4.21 |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O |
Saccharomyces cerevisiae |
first step in NAD+-(salvage) biosynthesis |
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.3.4.21 |
Saccharomyces cerevisiae |
- |
bakers´yeast |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
6.3.4.21 |
extraction, ammonium sulfate fractionation, chromatography on DEAE-cellulose and hydroxylapatite, 1000fold purified |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
6.3.4.21 |
2.3 |
- |
- |
Saccharomyces cerevisiae |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
6.3.4.21 |
-25°C, at least 1 month |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
6.3.4.21 |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O |
- |
Saccharomyces cerevisiae |
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate |
- |
? |
|
6.3.4.21 |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O |
first step in NAD+-(salvage) biosynthesis |
Saccharomyces cerevisiae |
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate |
- |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
6.3.4.21 |
37 |
- |
assay at |
Saccharomyces cerevisiae |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
6.3.4.21 |
50 |
- |
5 min: 95% loss of activity, MgATP2-, Mg-phosphoribose diphosphate, ATP, GTP or nicotinic acid protect, with 27%, 37%, 52%, 54% or 61% loss of activity, respectively. 10 min: 97% loss of activity, MgATP2-, Mg-5-phospho-alpha-D-ribose 1-diphosphate, ATP, GTP or nicotinic acid protect, with 43%, 52%, 60%, 63% or 69% loss of activity, respectively |
Saccharomyces cerevisiae |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
6.3.4.21 |
7.5 |
8.5 |
broad |
Saccharomyces cerevisiae |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
6.3.4.21 |
5.5 |
10 |
about half-maximal activity at pH 5.5 and 10 |
Saccharomyces cerevisiae |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
6.3.4.21 |
ATP |
1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed |
Saccharomyces cerevisiae |
|
6.3.4.21 |
ATP |
together with Mg2+ |
Saccharomyces cerevisiae |
|
6.3.4.21 |
CTP |
one third the activity of ATP |
Saccharomyces cerevisiae |
|
6.3.4.21 |
GTP |
shows almost as much activity as ATP |
Saccharomyces cerevisiae |
|
6.3.4.21 |
ITP |
shows almost as much activity as ATP |
Saccharomyces cerevisiae |
|
6.3.4.21 |
TTP |
not |
Saccharomyces cerevisiae |
|
6.3.4.21 |
UTP |
one third the activity of ATP |
Saccharomyces cerevisiae |
|