Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Imsande, J.
    Pathway of diphosphopyridine nucleotide biosynthesis in Escherichia coli (1961), J. Biol. Chem., 236, 1494-1497.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.21 GSH activation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.18 0.03
-
 nicotinate ribonucleotide 37°C, pH 7.5 Escherichia coli
2.7.7.18 0.5
-
 ATP 37°C, pH 7.5 Escherichia coli
6.3.4.21 0.002
-
 nicotinate
-
 Escherichia coli
6.3.4.21 0.03
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli
6.3.4.21 0.4
-
 ATP
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.18 Mg2+ requirement Escherichia coli
6.3.4.21 Mg2+ requirement Escherichia coli
6.3.4.21 Mg2+ maximal activity: 20 mM Escherichia coli
6.3.4.21 Mg2+ synergism with ATP Escherichia coli
6.3.4.21 NaF activation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.18 ATP + nicotinate ribonucleotide Escherichia coli
-
 diphosphate + deamido-NAD+
-
 r
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O Escherichia coli
-
 beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.18 Escherichia coli
-
 strains K-12 or B <3>
-
6.3.4.21 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.18 partial Escherichia coli
6.3.4.21 bacteria disruption by alumina grinding, protamine sulfate precipitation and chromatography on DEAE-cellulose Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.18 ATP + nicotinate ribonucleotide
-
 Escherichia coli diphosphate + deamido-NAD+
-
 r
2.7.7.18 ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Escherichia coli diphosphate + deamido-NAD+ i.e. nicotinic acid adenine dinucleotide r
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
 Escherichia coli beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
 Escherichia coli beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
6.3.4.21 nicotinic acid mononucleotide pyrophosphorylase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.18 37
-
 assay at Escherichia coli
6.3.4.21 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.18 7.5
-
-
 Escherichia coli
6.3.4.21 8.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.21 additional information
-
 active over a wide range Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.21 ATP activation Escherichia coli
6.3.4.21 ATP together with Mg2+ Escherichia coli
6.3.4.21 UTP
-
 Escherichia coli