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Literature summary extracted from

  • Fishman, P.H.; Bradley, R.M.; Henneberry, R.C.
    Butyrate-induced glycolipid biosynthesis in HeLa cells: properties of the induced sialyltransferase (1976), Arch. Biochem. Biophys., 172, 618-626.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.9 cardiolipin activation Homo sapiens
2.4.3.9 Cutscum activation, most potent activator Homo sapiens
2.4.3.9 Cutscum can be replaced by Triton CF-54, Triton X-100 or Triton CF-54/Tween 80, ratio 2:1, activation efficiency in descending order Homo sapiens
2.4.3.9 Nonidet P-40 slight activation Homo sapiens
2.4.3.9 Triton CF-54
-
 Homo sapiens
2.4.3.9 Triton CF-54/Tween 80 ratio 2:1, activation, about 50% as efficient as Cutscum Homo sapiens
2.4.3.9 Triton X-100 activation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.9 additional information not inhibited by EDTA Homo sapiens
2.4.3.9 Tris buffer weak Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.9 0.035
-
 lactosylceramide
-
 Homo sapiens
2.4.3.9 1.5
-
 CMP-N-acetylneuraminate
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.3.9 additional information no metal ion requirement Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.8 Homo sapiens
-
-
-
2.4.3.9 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.8 HeLa cell
-
 Homo sapiens
-
2.4.3.9 HeLa cell strain R, elevated enzyme level if cultured in butyrate containing media Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.9 0.0000045
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.8 CMP-N-acetylneuraminate + N-alpha-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Homo sapiens CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. disialosyllactosylceramide or ganglioside GD3 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Homo sapiens CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.3.9 100
-
 5 min, inactivation Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.9 6
-
-
 Homo sapiens