Literature summary extracted from
Hirooka, K.; Ohnuma, S.I.; Koike-Takeshita, A.; Koyama, T.; Nishino, T.
Mechanism of product chain length determination for heptaprenyl diphosphate synthase from Bacillus stearothermophilus (2000), Eur. J. Biochem., 267, 4520-4528.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.5.1.30 |
A79Y |
single replacement to the aromatic residue at the fourth or the fifth position before the first aspartate-rich motif (FARM), mainly yields a C20 product |
Bacillus subtilis |
2.5.1.30 |
I76G |
can catalyze condensations of isopentenyl diphosphate beyond the native chain length of C35. With farnesyl diphosphate as allylic substrate the mutant enzyme largely produces (all-trans)-octaprenyl diphosphate (C40, 21%) with a small amount of solanesyl diphosphate (C45, 5.3%). With geranylgeranyl diphosphate as allylic substrate the mutant enzyme yields solanesyl diphosphate (33%) as the main product with a large amount of (all-trans)-octaprenyl diphosphate (21%) |
Bacillus subtilis |
2.5.1.30 |
additional information |
elucidation of the mechanism of chain length determination of Bacillus stearothermophilus HepPS by site-directed mutagenesis |
Bacillus subtilis |
2.5.1.30 |
S80F |
single replacement to the aromatic residue at the fourth or the fifth position before the first aspartate-rich motif (FARM), mainly yields a C20 product |
Bacillus subtilis |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.30 |
Bacillus subtilis |
P31112 and P31114 |
P31112: subunit 1, P31114: subunit 2 |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.5.1.30 |
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate = 4 diphosphate + all-trans-heptaprenyl diphosphate |
a common mechanism controls the product chain length of both short-chain and medium-chain prenyl diphosphate synthases. In wild-type heptaprenyl diphosphate synthase, the prenyl chain can grow on the surface of the small residues at positions 79 and 80, and the elongation is precisely blocked at the length of C35 by Ile76 |
Bacillus subtilis |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.5.1.30 |
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate |
wild-type enzyme mainly produces heptaprenyl diphosphate (C35, molar ratio, 73%) and does not yield any product longer than heptaprenyl diphosphate |
Bacillus subtilis |
4 diphosphate + all-trans-heptaprenyl diphosphate |
- |
? |
|
2.5.1.30 |
geranylgeranyl diphosphate + isopentenyl diphosphate |
- |
Bacillus subtilis |
diphosphate + all-trans-heptaprenyl diphosphate + geranylfarnesyl diphosphate |
wild-type enzyme mainly yields geranylfarnesyl diphosphate (C25, 59%) with a significant amount of heptaprenyl diphosphate (37%) |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.5.1.30 |
HepPS |
- |
Bacillus subtilis |