Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Suzuki, Y.; Terai, Y.; Abe, S.
    Purification and some properties of riboflavin synthetase from Bacillus stearothermophilus ATCC 8005 (1978), Appl. Environ. Microbiol., 35, 258-263.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.9 additional information no requirement for a cofactor Geobacillus stearothermophilus

General Stability

EC Number General Stability Organism
2.5.1.9 stable at 26°C in 4 M urea for 18 h, 4 M urea completely inactivates at 55°C Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.9 5,5'-dithiobis(2-nitrobenzoate)
-
 Geobacillus stearothermophilus
2.5.1.9 6-Methyl-7-hydroxy-8-ribityllumazine
-
 Geobacillus stearothermophilus
2.5.1.9 Ca2+
-
 Geobacillus stearothermophilus
2.5.1.9 Cu2+
-
 Geobacillus stearothermophilus
2.5.1.9 Hg2+
-
 Geobacillus stearothermophilus
2.5.1.9 iodoacetamide
-
 Geobacillus stearothermophilus
2.5.1.9 Mg2+
-
 Geobacillus stearothermophilus
2.5.1.9 Mn2+
-
 Geobacillus stearothermophilus
2.5.1.9 additional information no effect by Ba2+, Co2+, EDTA Geobacillus stearothermophilus
2.5.1.9 p-chloromercuribenzoate
-
 Geobacillus stearothermophilus
2.5.1.9 Zn2+
-
 Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.9 additional information
-
 additional information Km is temperature-dependent Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.9 additional information no effect by Ba2+, Co2+ Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.9 345000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Geobacillus stearothermophilus
-
 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.9 Geobacillus stearothermophilus
-
 strain ATCC 8005
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.9
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.9 0.011
-
 purified enzyme Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
2.5.1.9 4°C, 6 months, no loss of activity Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
 Geobacillus stearothermophilus riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.9 95
-
-
 Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.9 50 95 50°C: about 10% of activity maximum, 80°C: about 30% of activity maximum, 95°C: activity maximum Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.9 60
-
 24 h, no loss of activity, without substrate Geobacillus stearothermophilus
2.5.1.9 85
-
 10 min, complete loss of activity Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.9 6.7 7.2
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.9 5.4 8.8 about 50% of activity maximum at pH 5.4 and 8.8 Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.9 6 10 26°C, 18 h, stable Geobacillus stearothermophilus
2.5.1.9 6.3 7.6 55°C, 18 h, stable Geobacillus stearothermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.9 0.18
-
 6-Methyl-7-hydroxy-8-ribityllumazine
-
 Geobacillus stearothermophilus