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Literature summary extracted from

  • Bacher, A.; Baur, R.; Eggers, U.; Harders, H.D.; Otto, M.K.; Schnepple, H.
    Riboflavin synthases of Bacillus subtilis. Purification and properties (1980), J. Biol. Chem., 255, 632-637.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.9 SO32- enhances activity Bacillus subtilis
2.5.1.9 SO32- enhances activity Saccharomyces cerevisiae
2.5.1.9 SO32- sodium sulfite Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.9 0.013
-
6,7-dimethyl-8-(1'-D-ribityl)lumazine heavy enzyme Bacillus subtilis
2.5.1.9 0.014
-
6,7-dimethyl-8-(1'-D-ribityl)lumazine light enzyme Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.9 23500
-
3 * 23500, light enzyme, SDS-PAGE, subunits not covalently linked Bacillus subtilis
2.5.1.9 70000
-
analytical ultracentrifugation Bacillus subtilis
2.5.1.9 70000
-
light enzyme Bacillus subtilis
2.5.1.9 100000
-
analytical ultracentrifugation Bacillus subtilis
2.5.1.9 100000
-
heavy riboflavin synthase Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Lactiplantibacillus plantarum
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Bacillus subtilis
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Escherichia coli
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Saccharomyces cerevisiae
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Priestia megaterium
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Moorella thermoacetica
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Bacillus licheniformis
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Paenibacillus polymyxa
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Streptomyces venezuelae
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Rhodococcus ruber
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Pseudomonas iodinum
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Escherichia coli B / ATCC 11303
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.9 Bacillus licheniformis
-
contains heavy and light form of the enzyme
-
2.5.1.9 Bacillus subtilis
-
heavy and light enzyme form
-
2.5.1.9 Bacillus subtilis
-
H94) (flavinogenic), H322 (flavinogenic), (H52, Rib-), (107,Rib-), ATCC 6051, ATCC 6633, B23
-
2.5.1.9 Bacillus subtilis
-
contains heavy and light form of the enzyme
-
2.5.1.9 Escherichia coli
-
B
-
2.5.1.9 Escherichia coli B / ATCC 11303
-
B
-
2.5.1.9 Lactiplantibacillus plantarum
-
-
-
2.5.1.9 Moorella thermoacetica
-
contains heavy and light form of the enzyme
-
2.5.1.9 Paenibacillus polymyxa
-
contains heavy and light form of the enzyme
-
2.5.1.9 Priestia megaterium
-
contains heavy and light form of the enzyme
-
2.5.1.9 Pseudomonas iodinum
-
-
-
2.5.1.9 Rhodococcus ruber
-
-
-
2.5.1.9 Saccharomyces cerevisiae
-
-
-
2.5.1.9 Streptomyces venezuelae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.9 heavy enzyme Bacillus subtilis
2.5.1.9 light enzyme Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.9 0.033
-
heavy enzyme Bacillus subtilis
2.5.1.9 0.833
-
light enzyme Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Lactiplantibacillus plantarum riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Bacillus subtilis riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Escherichia coli riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Saccharomyces cerevisiae riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Priestia megaterium riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Moorella thermoacetica riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Bacillus licheniformis riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Paenibacillus polymyxa riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Streptomyces venezuelae riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Rhodococcus ruber riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Pseudomonas iodinum riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?
2.5.1.9 6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Escherichia coli B / ATCC 11303 riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.9 trimer
-
Escherichia coli
2.5.1.9 trimer 3 * 23500, light enzyme, SDS-PAGE, subunits not covalently linked Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.9 1.2
-
6,7-dimethyl-8-(1'-D-ribityl)lumazine heavy enzyme, Bacillus subtilis H94 Bacillus subtilis
2.5.1.9 2
-
6,7-dimethyl-8-(1'-D-ribityl)lumazine light enzyme, Bacillus subtilis H94 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.9 7.4
-
light enzyme, the pH-optimum of the heavy enzyme is similar, detailed measurement is not possible because of instability at elevated pH Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.9 additional information
-
heavy enzyme is instable at elevated pH Bacillus subtilis