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Literature summary extracted from

  • Cacciapuoti, G.; Bertoldo, C.; Brio, A.; Zappia, V.; Porcelli, M.
    Purification and characterization of 5'-methylthioadenosine phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus. Substrate specificity and primary structure analysis (2003), Extremophiles, 7, 159-168.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.28 dithiothreitol 0.8 M, reduction of thermostability Pyrococcus furiosus
2.4.2.28 enzyme is extremely stable to proteolytic cleavage Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.28 dithiothreitol 0.8 M, reduction of thermostability Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.28 0.109
-
 adenosine
-
 Pyrococcus furiosus
2.4.2.28 0.147
-
 5'-methylthioadenosine
-
 Pyrococcus furiosus
2.4.2.28 0.916
-
 guanosine
-
 Pyrococcus furiosus
2.4.2.28 0.963
-
 Inosine
-
 Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.28 30000
-
 6 * 30000, SDS-PAGE Pyrococcus furiosus
2.4.2.28 180000
-
 gel filtration Pyrococcus furiosus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.4.2.28 1-propanol at 50%: 88% remaining activity after 60 min at 50°C, 58% remaining activity after 60 min at 70°C, 22% remaining activity after 60 min at 90°C Pyrococcus furiosus
2.4.2.28 acetonitrile at 50%: stable at 90°C for 30 min, loss of 62% activity after 60 min Pyrococcus furiosus
2.4.2.28 dimethylformamide at 50%: 48% remaining activity after 60 min at 50°C, 5% remaining activity after 60 min at 70°C, no activity at 90°C Pyrococcus furiosus
2.4.2.28 Ethanol at 50%: 90% remaining activity after 60 min at 50°C, 65% remaining activity after 60 min at 70°C, 30% remaining activity after 60 min at 90°C Pyrococcus furiosus
2.4.2.28 Methanol at 50%: stable at 90°C for 30 min, loss of 77% activity after 60 min Pyrococcus furiosus
2.4.2.28 tetrahydrofuran at 50%: 77% remaining activity after 60 min at 50°C, 50% remaining activity after 60 min at 70°C, 3% remaining activity after 60 min at 90°C Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.28
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.28 6.35
-
 purified enzyme Pyrococcus furiosus

Storage Stability

EC Number Storage Stability Organism
2.4.2.28 20°C, Tris-HCl 10 mM, pH 7.4, purified enzyme, stable for at least 1 year Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 5'-methylthioadenosine + phosphate
-
 Pyrococcus furiosus adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 adenosine + phosphate
-
 Pyrococcus furiosus adenine + D-ribose 1-phosphate
-
 ?
2.4.2.28 guanosine + phosphate
-
 Pyrococcus furiosus guanine + D-ribose 1-phosphate
-
 ?
2.4.2.28 inosine + phosphate
-
 Pyrococcus furiosus hypoxanthine + D-ribose 1-phosphate
-
 ?
2.4.2.28 additional information 6-amino purine nucleosides are the preferred substrates, substrate specificity Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.28 hexamer 6 * 30000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.28 80
-
 assay at Pyrococcus furiosus
2.4.2.28 125
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.28 70 160
-
 Pyrococcus furiosus
2.4.2.28 86 95 2 different activation energy-dependent processes occur below and above 100°C Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.28 100
-
 5 h, 98% remaining activity Pyrococcus furiosus
2.4.2.28 130
-
 half-life: 43 min Pyrococcus furiosus
2.4.2.28 137
-
 melting temperature Pyrococcus furiosus
2.4.2.28 139
-
 melting temperature in presence of 100 mM phosphate Pyrococcus furiosus
2.4.2.28 140
-
 half-life: 13 min Pyrococcus furiosus
2.4.2.28 145
-
 half-life: 5 min Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.28 7.31
-
 guanosine
-
 Pyrococcus furiosus
2.4.2.28 9.38
-
 Inosine
-
 Pyrococcus furiosus
2.4.2.28 22.8
-
 adenosine
-
 Pyrococcus furiosus
2.4.2.28 24.5
-
 5'-methylthioadenosine
-
 Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.28 7.4
-
 assay at Pyrococcus furiosus