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Literature summary extracted from

  • Gomez, R.; Goodman, L.E.; Tripathy, S.K.; O'Rourke, E.; Manne, V.; Tamanoi, F.
    Purified yeast protein farnesyltransferase is structurally and functionally similar to its mammalian counterpart (1993), Biochem. J., 289, 25-31.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.5.1.58 medicine
-
 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.58 additional information enzyme inhibited by peptides containing the C-terminal CAAX sequence Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.58 0.0051
-
 GST-CIIS
-
 Saccharomyces cerevisiae
2.5.1.58 0.0081
-
 farnesyl diphosphate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.58 Mg2+ required Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.58 34000
-
 1 * 43000 + 1 * 34000 Saccharomyces cerevisiae
2.5.1.58 43000
-
 1 * 43000 + 1 * 34000 Saccharomyces cerevisiae
2.5.1.58 90000
-
-
 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.58 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.58
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.58 farnesyl diphosphate + GST-CIIL
-
 Saccharomyces cerevisiae diphosphate + S-farnesyl-GST-Cys-Ile-Ile-Ser
-
 ?
2.5.1.58 farnesyl diphosphate + GST-CIIS GST-CIIS is glutathione S-transferase fused to the C-terminal 12 amino acids of yeast RAS2 protein Saccharomyces cerevisiae ?
-
 ?
2.5.1.58 farnesyl diphosphate + protein-cysteine prenylation, farnesylation, substrates are Ras, nuclear lamins, transducin gamma subunit, protein substrate motif: Cys-aliphatic-aliphatic-X, X: M, S, Q, A, F Saccharomyces cerevisiae diphosphate + S-farnesyl protein
-
 ?
2.5.1.58 farnesyl diphosphate + [GST]-Cys-Ile-Ile-Ser
-
 Saccharomyces cerevisiae diphosphate + S-farnesyl-[GST]-Cys-Ile-Ile-Ser
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.58 heterodimer 1 * 43000 + 1 * 34000 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.58 50
-
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.58 7.8
-
-
 Saccharomyces cerevisiae