EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.31 | Triton X-100 | no activity in absence | Escherichia coli | |
2.5.1.31 | Triton X-100 | no activity in the activation by Triton X-100 is almost saturated at a concentration of 0.02% | Escherichia coli | |
2.5.1.90 | Triton X-100 | addition of 0.1% and 0.5% Triton X-100 activates the enzyme by 3.9- and 4.7fold, respectively | Escherichia coli K-12 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.31 | 0.0007 | - |
farnesyl diphosphate | pH 7.5, 30°C | Escherichia coli | |
2.5.1.31 | 0.029 | - |
isopentenyl diphosphate | pH 7.5, 30°C | Escherichia coli | |
2.5.1.90 | 0.00018 | - |
isopentenyl diphosphate | pH 7.5, 30°C | Escherichia coli K-12 | |
2.5.1.90 | 0.00025 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 30°C | Escherichia coli K-12 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.31 | Mg2+ | Mg2+ or Mn2+ required. Optimal concentration for Mg2+ is 1.0 mM | Escherichia coli | |
2.5.1.31 | Mg2+ | or Mn2+ required. Optimal concentration 1.0 mM | Escherichia coli | |
2.5.1.31 | Mn2+ | or Mg2+ required | Escherichia coli | |
2.5.1.31 | Mn2+ | Mn2+ or Mg2+ required | Escherichia coli | |
2.5.1.31 | Mn2+ | maximal stimulation at 0.05-0.1 mM, Mg2+ or Mn2+ required | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.31 | Escherichia coli | - |
- |
- |
2.5.1.90 | Escherichia coli K-12 | P0AD57 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.31 | partial | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.5.1.31 | -20°C, activity in enzyme preparation is stable for several weeks | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.31 | farnesyl diphosphate + 8 isopentenyl diphosphate | neither dimethylallyl diphosphate nor geranyl diphosphate act as priming substrate for the enzyme | Escherichia coli | 8 diphosphate + undecaprenyl diphosphate | - |
? | |
2.5.1.31 | isopentenyl diphosphate + farnesyl diphosphate | - |
Escherichia coli | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | with 0.02% Triton X-100 the main product is the C55-compound, C50-compound is the minor product. With 0.1% Triton X-100 the C50-polyprenyl diphosphate is the main product, the C55-polyprenyl diphosphate and the C45-polyprenyl diphosphate are minor products. With 0.5% Triton X-100 the C50-polyprenyl diphosphate compound and the C45-compound are the main products, the C55-compound is not formed | ? | |
2.5.1.90 | (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | - |
Escherichia coli K-12 | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
2.5.1.90 | additional information | no substrate: dimethylallyl diphosphate, geranyl diphosphate | Escherichia coli K-12 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.31 | undecaprenyl pyrophosphate synthetase | - |
Escherichia coli |
2.5.1.31 | UPP synthetase | - |
Escherichia coli |
2.5.1.90 | octaprenyl pyrophosphate | - |
- |
2.5.1.90 | OPP synthetase | - |
- |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.31 | 30 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.31 | 7.5 | 9 | - |
Escherichia coli |