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Literature summary extracted from

  • Fujisaki, S.; Nishino, T.; Katasuki, H.
    Isoprenoid synthesis in Escherichia coli. Separation and partial purification of four enzymes involved in the synthesis (1986), J. Biochem., 99, 1327-1337.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.31 Triton X-100 no activity in absence Escherichia coli
2.5.1.31 Triton X-100 no activity in the activation by Triton X-100 is almost saturated at a concentration of 0.02% Escherichia coli
2.5.1.90 Triton X-100 addition of 0.1% and 0.5% Triton X-100 activates the enzyme by 3.9- and 4.7fold, respectively Escherichia coli K-12

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.31 0.0007
-
farnesyl diphosphate pH 7.5, 30°C Escherichia coli
2.5.1.31 0.029
-
isopentenyl diphosphate pH 7.5, 30°C Escherichia coli
2.5.1.90 0.00018
-
isopentenyl diphosphate pH 7.5, 30°C Escherichia coli K-12
2.5.1.90 0.00025
-
(2E,6E)-farnesyl diphosphate pH 7.5, 30°C Escherichia coli K-12

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.31 Mg2+ Mg2+ or Mn2+ required. Optimal concentration for Mg2+ is 1.0 mM Escherichia coli
2.5.1.31 Mg2+ or Mn2+ required. Optimal concentration 1.0 mM Escherichia coli
2.5.1.31 Mn2+ or Mg2+ required Escherichia coli
2.5.1.31 Mn2+ Mn2+ or Mg2+ required Escherichia coli
2.5.1.31 Mn2+ maximal stimulation at 0.05-0.1 mM, Mg2+ or Mn2+ required Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.31 Escherichia coli
-
-
-
2.5.1.90 Escherichia coli K-12 P0AD57
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.31 partial Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.5.1.31 -20°C, activity in enzyme preparation is stable for several weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.31 farnesyl diphosphate + 8 isopentenyl diphosphate neither dimethylallyl diphosphate nor geranyl diphosphate act as priming substrate for the enzyme Escherichia coli 8 diphosphate + undecaprenyl diphosphate
-
?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate
-
Escherichia coli C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate with 0.02% Triton X-100 the main product is the C55-compound, C50-compound is the minor product. With 0.1% Triton X-100 the C50-polyprenyl diphosphate is the main product, the C55-polyprenyl diphosphate and the C45-polyprenyl diphosphate are minor products. With 0.5% Triton X-100 the C50-polyprenyl diphosphate compound and the C45-compound are the main products, the C55-compound is not formed ?
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
-
Escherichia coli K-12 5 diphosphate + all-trans-octaprenyl diphosphate
-
?
2.5.1.90 additional information no substrate: dimethylallyl diphosphate, geranyl diphosphate Escherichia coli K-12 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.31 undecaprenyl pyrophosphate synthetase
-
Escherichia coli
2.5.1.31 UPP synthetase
-
Escherichia coli
2.5.1.90 octaprenyl pyrophosphate
-
-
2.5.1.90 OPP synthetase
-
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.31 30
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.31 7.5 9
-
Escherichia coli