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Literature summary extracted from

  • Baba, T.; Allen, C.M.
    Prenyl transferases from Micrococcus luteus: characterization of undecaprenyl pyrophosphate synthetase (1980), Arch. Biochem. Biophys., 200, 474-484.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.31 cardiolipin poor activator Micrococcus luteus
2.5.1.31 deoxycholate poor activator Micrococcus luteus
2.5.1.31 lecithin from egg, stimulates Micrococcus luteus
2.5.1.31 lecithin 14fold stimulation at 2 mM Micrococcus luteus
2.5.1.31 additional information enzyme is inactive in absence of added effectors Micrococcus luteus
2.5.1.31 Phospholipid phospholipid extract from Micrococcus luteus stimulates Micrococcus luteus
2.5.1.31 Triton X-100 stimulates Micrococcus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.31 0.007
-
 (E,E)-farnesyl diphosphate pH 7.4, 35°C Micrococcus luteus
2.5.1.31 0.008
-
 (E,E,E)-geranylgeranyl diphosphate pH 7.5, 35°C Micrococcus luteus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.31 soluble
-
 Micrococcus luteus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.31 Co2+ enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ Micrococcus luteus
2.5.1.31 Mg2+ maximal stimulation at 0.1 mM Micrococcus luteus
2.5.1.31 Mn2+ enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ Micrococcus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.31 47000 49000 gel filtration Micrococcus luteus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.31 Micrococcus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.31
-
 Micrococcus luteus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.31 0.004066
-
-
 Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.31 (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + DELTA3-isopentenyl diphosphate
-
 Micrococcus luteus ?
-
 ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate cis,trans-farnesyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate trans,trans-farnesyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + geranyl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + geranylgeranyl diphosphate trans,trans,trans-geranylgeranyl diphosphate and cis,trans,trans-geranylgeranyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + neryl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 additional information reactivities of the allylic substrates increase with chain length: C10, C15, C20. Each new isoprene unit added has a cis-configuration Micrococcus luteus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.31 7.5
-
-
 Micrococcus luteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.31 7.5 9 pH 7.5: activity maximum, pH 9.0: about 80% of maximal activity Micrococcus luteus