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Literature summary extracted from

  • Allen, C.M.
    Purification and characterization of undecaprenylpyrophosphate synthetase (1985), Methods Enzymol., 110, 281-299.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.31 cardiolipin weak stimulation Micrococcus luteus
2.5.1.31 cardiolipin Lactobacillus plantarum, Escherichia coli, and bovine cardiolipin. Good activator in absence of detergent Lactiplantibacillus plantarum
2.5.1.31 cetyl sulfate half-maximal activation at 0.04 mM Lactiplantibacillus plantarum
2.5.1.31 deoxycholate weak stimulation Bacillus subtilis
2.5.1.31 deoxycholate weak stimulation Micrococcus luteus
2.5.1.31 deoxycholate half-maximal activation at 0.33 mM Lactiplantibacillus plantarum
2.5.1.31 Dodecyl sulfate half-maximal activation at 0.075 mM Lactiplantibacillus plantarum
2.5.1.31 lecithin weak stimulation Bacillus subtilis
2.5.1.31 lecithin from egg, stimulates Micrococcus luteus
2.5.1.31 oleic acid good activator in absence of detergent Lactiplantibacillus plantarum
2.5.1.31 phosphatidic acid from egg, good activator in absence of detergent Lactiplantibacillus plantarum
2.5.1.31 Triton X-100 stimulates Micrococcus luteus
2.5.1.31 Triton X-100 strong stimulation Bacillus subtilis
2.5.1.31 Triton X-100 half-maximal activation at 3.4 mM Lactiplantibacillus plantarum
2.5.1.31 Tween 80 strong stimulation Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.31 1,2-Cyclohexanedione
-
 Lactiplantibacillus plantarum
2.5.1.31 Butanedione
-
 Lactiplantibacillus plantarum
2.5.1.31 iodoacetate
-
 Lactiplantibacillus plantarum
2.5.1.31 additional information no inhibition by phosphate Lactiplantibacillus plantarum
2.5.1.31 Phenylglyoxal
-
 Lactiplantibacillus plantarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.31 0.00013
-
 (E,E)-farnesyl diphosphate pH 7.5, 35°C Lactiplantibacillus plantarum
2.5.1.31 0.00192
-
 isopentenyl diphosphate pH 7.5, 35°C Lactiplantibacillus plantarum
2.5.1.31 0.008
-
 (E,E)-farnesyl diphosphate
-
 Micrococcus luteus
2.5.1.31 0.0091
-
 (E,E)-farnesyl diphosphate
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.31 Co2+ divalent cation required Lactiplantibacillus plantarum
2.5.1.31 Co2+ divalent cation required Bacillus subtilis
2.5.1.31 Co2+ divalent cation required Micrococcus luteus
2.5.1.31 Co2+ enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ Micrococcus luteus
2.5.1.31 Co2+ enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+ Lactiplantibacillus plantarum
2.5.1.31 K+ stimulates in presence of Mg2+ Lactiplantibacillus plantarum
2.5.1.31 K+ stimulates in presence of Mg2+ Bacillus subtilis
2.5.1.31 Mg2+ divalent cation required Lactiplantibacillus plantarum
2.5.1.31 Mg2+ divalent cation required Bacillus subtilis
2.5.1.31 Mg2+ divalent cation required Micrococcus luteus
2.5.1.31 Mg2+ enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ Micrococcus luteus
2.5.1.31 Mg2+ enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+ Lactiplantibacillus plantarum
2.5.1.31 Mg2+ best stimulation by Mg2+. Mn2+ and Zn2+ may partially replace Mn2+ Bacillus subtilis
2.5.1.31 Mn2+ can partially replace Mg2+ Bacillus subtilis
2.5.1.31 Mn2+ enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ Micrococcus luteus
2.5.1.31 Mn2+ enzyme is equally stimulated by 0.2 mM Mg2+, 0.2 mM Co2+ or 0.1 mM Mn2+ Lactiplantibacillus plantarum
2.5.1.31 Mn2+ best stimulation by Mg2+. Mn2+ and Zn2+ may partially replace Mn2+ Bacillus subtilis
2.5.1.31 Na+ stimulates in presence of Mg2+ Lactiplantibacillus plantarum
2.5.1.31 Na+ stimulates in presence of Mg2+ Bacillus subtilis
2.5.1.31 NH4+ stimulates in presence of Mg2+ Lactiplantibacillus plantarum
2.5.1.31 NH4+ stimulates in presence of Mg2+ Bacillus subtilis
2.5.1.31 Zn2+
-
 Lactiplantibacillus plantarum
2.5.1.31 Zn2+
-
 Micrococcus luteus
2.5.1.31 Zn2+ can partially replace Mg2+ Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.31 30000
-
 2 * 30000, SDS-PAGE Lactiplantibacillus plantarum
2.5.1.31 52000 58000 sucrose density gradient centrifugation Lactiplantibacillus plantarum
2.5.1.31 53000 60000 gel filtration Lactiplantibacillus plantarum
2.5.1.31 56000
-
 gel filtration, sucrose density gradient centrifugation Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.31 additional information Lactiplantibacillus plantarum involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components ?
-
 ?
2.5.1.31 additional information Bacillus subtilis involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components ?
-
 ?
2.5.1.31 additional information Micrococcus luteus involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.31 Bacillus subtilis
-
-
-
2.5.1.31 Lactiplantibacillus plantarum
-
 ATCC 8014
-
2.5.1.31 Micrococcus luteus
-
-
-
2.5.1.31 Salmonella newington
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.31
-
 Lactiplantibacillus plantarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.31 additional information
-
-
 Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.31 (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Bacillus subtilis 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
2.5.1.31 (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate
-
 Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate
-
 Salmonella newington C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate trans,trans-farnesyl diphosphate and cis,trans-farnesyl diphosphate Lactiplantibacillus plantarum C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate trans,trans-farnesyl diphosphate Lactiplantibacillus plantarum C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate trans,trans-farnesyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + geranyl diphosphate
-
 Lactiplantibacillus plantarum C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + geranyl diphosphate
-
 Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + geranyl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + geranylgeranyl diphosphate
-
 Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + geranylgeranyl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + geranylgeranyl diphosphate trans,trans,trans-geranylgeranyl diphosphate Lactiplantibacillus plantarum C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + geranylneryl diphosphate cis,trans,trans-geranylneryl diphosphate Lactiplantibacillus plantarum ?
-
 ?
2.5.1.31 isopentenyl diphosphate + neryl diphosphate
-
 Lactiplantibacillus plantarum C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + neryl diphosphate
-
 Bacillus subtilis C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 isopentenyl diphosphate + neryl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
2.5.1.31 additional information involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components Lactiplantibacillus plantarum ?
-
 ?
2.5.1.31 additional information involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components Bacillus subtilis ?
-
 ?
2.5.1.31 additional information involved in the biosynthesis of long-chain polyprenyl diphosphate required as a carbohydrate carrier in the biosynthesis of a variety of bacterial cell envelope components Micrococcus luteus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.31 dimer 2 * 30000, SDS-PAGE Lactiplantibacillus plantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.31 7.5
-
-
 Micrococcus luteus
2.5.1.31 7.5
-
 and a second optimum at pH 10.2, that is 40% of the optimum at pH 7.5 Lactiplantibacillus plantarum
2.5.1.31 8.5
-
-
 Bacillus subtilis
2.5.1.31 10.2
-
 and a second optimum at pH 7.5 that is higher than the optimum at pH 10.2 Lactiplantibacillus plantarum