Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fujii, H.; Koyama, T.; Ogura, K.
    Hexaprenyl pyrophosphate synthetase from Micrococcus luteus B-P 26. Separation of two essential components (1982), J. Biol. Chem., 257, 14610-14612.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.83 20000
-
 x * 20000 (component A), + x * 60000 (component B), during the course of purification the enzyme is resolved into two components (A and B), each of which has no catalytic activity but restore the hexaprenyl diphosphate synthase activity when combined with each other, gel filtration Micrococcus luteus
2.5.1.83 60000
-
 x * 20000 (component A), + x * 60000 (component B), during the course of purification the enzyme is resolved into two components (A and B), each of which has no catalytic activity but restore the hexaprenyl diphosphate synthase activity when combined with each other, gel filtration Micrococcus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.83 (2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate Micrococcus luteus the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate 3 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.83 (2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate Micrococcus luteus B-P 26 the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate 3 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.83 Micrococcus luteus O66127 and O66129 O66127: component A of hexaprenyl diphosphate synthase, O66129: component B of hexaprenyl diphosphate synthase
-
2.5.1.83 Micrococcus luteus B-P 26 O66127 and O66129 O66127: component A of hexaprenyl diphosphate synthase, O66129: component B of hexaprenyl diphosphate synthase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.83 during the course of purification the enzyme is resolved into two components (A and B), each of which has no catalytic activity but restore the hexaprenyl pyrophosphate synthetase activity when combined with each other Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.83 (2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate Micrococcus luteus 3 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.83 (2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate Micrococcus luteus B-P 26 3 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.83 geranylgeranyl diphosphate + 2 isopentenyl diphosphate the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate Micrococcus luteus 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.83 geranylgeranyl diphosphate + 2 isopentenyl diphosphate the enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate (activity is 3fold higher) as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate Micrococcus luteus B-P 26 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.83 ? x * 20000 (component A), + x * 60000 (component B), during the course of purification the enzyme is resolved into two components (A and B), each of which has no catalytic activity but restore the hexaprenyl diphosphate synthase activity when combined with each other, gel filtration Micrococcus luteus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.83 hexaprenyl pyrophosphate synthetase
-
 Micrococcus luteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.83 additional information
-
 component A is more stable against heat treatment than component B. The former retains 75% of its original activity for restoration after a 5 min treatment at 50°C while the latter loses 75% of its activity Micrococcus luteus