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Literature summary extracted from

  • Gebler, J.C.; Poulter, C.D.
    Purification and characterization of dimethylallyl tryptophan synthase from Claviceps purpurea (1992), Arch. Biochem. Biophys., 296, 308-313.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.34 0.008
-
 dimethylallyl diphosphate pH 7.8, 30°C, in presence of 4 mM CaCl2 Claviceps purpurea
2.5.1.34 0.012
-
 L-tryptophan pH 7.8, 30°C, in presence of 4 mM MgCl2 Claviceps purpurea
2.5.1.34 0.014
-
 dimethylallyl diphosphate
-
 Claviceps purpurea
2.5.1.34 0.017
-
 L-tryptophan pH 7.8, 30°C, in presence of 4 mM CaCl2 Claviceps purpurea
2.5.1.34 0.04
-
 L-tryptophan pH 7.8, 30°C Claviceps purpurea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.34 Ca2+ maximal stimulation at 4-10 mM Claviceps purpurea
2.5.1.34 Mg2+ maximal stimulation at 4-10 mM Claviceps purpurea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.34 53000
-
 alpha2, 2 * 53000, SDS-PAGE Claviceps purpurea
2.5.1.34 105000
-
 gel filtration Claviceps purpurea

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.34 Claviceps purpurea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.34
-
 Claviceps purpurea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.34 mycelium
-
 Claviceps purpurea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.34 0.5
-
-
 Claviceps purpurea

Storage Stability

EC Number Storage Stability Organism
2.5.1.34 -20°C, glycerol-containing buffer, stable for more than 6 months Claviceps purpurea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
 Claviceps purpurea diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.34 dimer alpha2, 2 * 53000, SDS-PAGE Claviceps purpurea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.34 7.5
-
-
 Claviceps purpurea