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Literature summary extracted from

  • Guggenheim, S.; Flavin, M.
    Cystathionine gamma-synthase from Salmonella. Beta elimination and replacement reactions and inhibition by O-succinylserine (1969), J. Biol. Chem., 244, 3722-3727.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.48 dithiothreitol 10% inhibition of beta-elimination from O-succinyl-L-serine Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.48 L-cysteine complete inhibition of gamma-elimination and beta-elimination Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.48 L-homocysteine partial inhibition of beta-elimination and gamma-elimination Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.48 0.7
-
 O-Succinyl-L-serine final, inhibited reaction phase Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.48 1.2
-
 O-Succinyl-L-serine initial reaction phase Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.48 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.48
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.48 18.7
-
 O-succinyl-L-homoserine + L-cysteine, gamma-replacement reaction Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.48 O-succinyl-L-homoserine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium succinate + 2-oxobutyrate + NH3
-
 ?
2.5.1.48 O-succinyl-L-homoserine + L-cysteine
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-cystathionine + succinate
-
 ?
2.5.1.48 O-succinyl-L-serine + H2O beta-elimination Salmonella enterica subsp. enterica serovar Typhimurium succinate + pyruvate + NH3
-
 ?
2.5.1.48 O-succinyl-L-serine + L-cysteine
-
 Salmonella enterica subsp. enterica serovar Typhimurium lanthionine + succinate
-
 ?
2.5.1.48 O-succinyl-L-serine + L-homocysteine beta-replacement, very low reaction rate Salmonella enterica subsp. enterica serovar Typhimurium succinate + L-cystathionine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.48 0.133
-
 O-Succinyl-L-serine gamma-replacement reaction, pH 8.2, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.48 1.5
-
 O-Succinyl-L-serine gamma-elimination reaction, final, inhibited reaction phase, pH 7.3, 30°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.48 3.83
-
 O-Succinyl-L-serine gamma-elimination reaction, initial reaction phase, pH 7.3, 30°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.48 4
-
 O-succinyl-L-homoserine gamma-elimination reaction, pH 7.3, 30°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.48 12.7
-
 O-succinyl-L-homoserine gamma-elimination reaction , pH 8.2, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.48 63.3
-
 O-succinyl-L-homoserine gamma-replacement reaction, pH 8.2, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.48 8.2
-
 O-succinyl-L-homoserine + H2O Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.48 6.5
-
 O-succinyl-L-homoserine + H2O, 12% of the reaction rate at pH 8.2 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.48 6.5
-
 O-succinyl-L-serine + H2O, 28% of the reaction rate at pH 8.2 Salmonella enterica subsp. enterica serovar Typhimurium