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Literature summary extracted from

  • Ray, P.
    Purification and characterization of 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli (1980), J. Bacteriol., 141, 635-644.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.5.1.55 dialysis against 10 mM EDTA in 50 mM Tris-HCl buffer, pH 7.3, inactivates Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.55 Cd2+
-
 Escherichia coli
2.5.1.55 Cu2+
-
 Escherichia coli
2.5.1.55 D-ribose 5-phosphate weak, competitive Escherichia coli
2.5.1.55 Hg2+ reversed by dithiothreitol Escherichia coli
2.5.1.55 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.55 0.006
-
 phosphoenolpyruvate pH 7.3, 37°C Escherichia coli
2.5.1.55 0.02
-
 D-arabinose 5-phosphate pH 7.3, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.55 Ba2+ 1 mM, 5-10% stimulation Escherichia coli
2.5.1.55 Fe3+ 1 mM, 5-10% stimulation Escherichia coli
2.5.1.55 Li+ 1 mM, 5-10% stimulation Escherichia coli
2.5.1.55 Mg2+ 1 mM, 5-10% stimulation Escherichia coli
2.5.1.55 Mn2+ 1 mM, 5-10% stimulation Escherichia coli
2.5.1.55 additional information no metal requirement Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.55 32000
-
 3 * 32000, SDS-PAGE Escherichia coli
2.5.1.55 90000
-
 gel filtration, non-denaturing PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Escherichia coli
-
-
-
2.5.1.55 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.55
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.55 3.7
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate
-
 Escherichia coli 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
 ?
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate
-
 Escherichia coli B / ATCC 11303 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.55 trimer 3 * 32000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.55 45
-
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.55 45
-
 15 min, 30% loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.55 4 6 and a second optimum at pH 9.0 Escherichia coli
2.5.1.55 9
-
 and a second optimum at pH 4.0-6.0 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.55 1
-
 D-ribose 5-phosphate pH 7.3, 37°C Escherichia coli