EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.47 | 5,5'-dithiobis(2-nitrobenzoic acid) | 1 mM, 92% inactivation | Thermus thermophilus | |
2.5.1.47 | hydroxylamine | 10 mM, 71.2% inhibition | Thermus thermophilus | |
2.5.1.47 | KCN | 15.6% inhibition by 1 mM | Thermus thermophilus | |
2.5.1.47 | Monoiodoacetic acid | 1 mM, complete inactivation | Thermus thermophilus | |
2.5.1.47 | phenylhydrazine | 73% inhibition by 1 mM, 97.4% inhibition by 10 mM | Thermus thermophilus | |
2.5.1.47 | Semicarbazide | 13% inhibition by 1 mM, 38.6% inhibition by 10 mM | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.47 | 0.05 | - |
H2S | pH 7.8, 50°C | Thermus thermophilus | |
2.5.1.47 | 4.8 | - |
O-acetyl-L-Ser | pH 7.8, 50°C | Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 34000 | - |
2 * 34000, SDS-PAGE | Thermus thermophilus |
2.5.1.47 | 68000 | - |
gel filtration | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.47 | Thermus thermophilus | - |
HB8 | - |
2.5.1.47 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
HB8 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.47 | - |
Thermus thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 337.4 | - |
- |
Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.47 | chloroalanine + sulfide | beta-chloroalanine, 6% of the activity with O-acetyl-L-Ser | Thermus thermophilus | cysteine + chloride | - |
r | |
2.5.1.47 | chloroalanine + sulfide | beta-chloroalanine, 6% of the activity with O-acetyl-L-Ser | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | cysteine + chloride | - |
r | |
2.5.1.47 | L-homoserine + sulfide | 1.6% of the activity with O-acetyl-L-serine | Thermus thermophilus | ? | - |
? | |
2.5.1.47 | O-acetyl-L-Ser + H2S | - |
Thermus thermophilus | L-Cys + acetate | - |
? | |
2.5.1.47 | O-acetyl-L-Ser + H2S | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-Cys + acetate | - |
? | |
2.5.1.47 | O-acetyl-L-serine | - |
Thermus thermophilus | L-cysteine + acetate | - |
? | |
2.5.1.47 | O-acetylhomoserine + H2S | 2.4% of the activity with O-acetyl-L-Ser | Thermus thermophilus | homocysteine + ? | - |
? | |
2.5.1.47 | O-acetylhomoserine + H2S | 2.4% of the activity with O-acetyl-L-Ser | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | homocysteine + ? | - |
? | |
2.5.1.47 | O-diazoacetyl-L-serine + sulfide | 44% of the activity with O-acetyl-Ser | Thermus thermophilus | ? | - |
? | |
2.5.1.47 | O-diazoacetyl-L-serine + sulfide | 44% of the activity with O-acetyl-Ser | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
2.5.1.47 | O-succinyl-L-homoserine + sulfide | 3.6% of the activity with O-acetyl-L-serine | Thermus thermophilus | ? | - |
? | |
2.5.1.47 | O-succinyl-L-homoserine + sulfide | 3.6% of the activity with O-acetyl-L-serine | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
2.5.1.47 | Ser + sulfide | 1.8% of the activity with O-acetyl-L-serine | Thermus thermophilus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.47 | dimer | 2 * 34000, SDS-PAGE | Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 55 | - |
- |
Thermus thermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 35 | 67 | about 50% of maximal activity at 35°C and at 67°C | Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 70 | - |
pH 7.8, 60 min, stable | Thermus thermophilus |
2.5.1.47 | 80 | - |
60 min, 10 mM dithiothreitol, less than 10% of activity | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 7 | - |
- |
Thermus thermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 5.5 | 8 | about 50% of maximal activity at pH 5.5 and pH 8.0 | Thermus thermophilus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 8 | 12 | 50°C, 30 min, stable | Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.47 | pyridoxal 5'-phosphate | Km of the cofactor at pH 8.0: 0.029 mM | Thermus thermophilus |