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Literature summary extracted from

  • Ikegami, F.; Itagaki, S.; Murakoshi, I.
    Purification and characterization of two forms of cysteine synthase from Allium tuberosum (1993), Phytochemistry, 32, 31-34.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 9
-
 O-acetyl-L-Ser isoenzyme 2 Allium tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 36000
-
 2 * 36000, isoenzyme 2, SDS-PAGE Allium tuberosum
2.5.1.47 40000
-
 2 * 40000, isoenzyme 1, SDS-PAGE Allium tuberosum
2.5.1.47 73000
-
 isoenzyme 2, gel filtration Allium tuberosum
2.5.1.47 81000
-
 isoenzyme 1, gel filtration Allium tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Allium tuberosum
-
 two isoforms, immunologically distinct
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Allium tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 leaf
-
 Allium tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 1.01
-
 isoenzyme 2 Allium tuberosum
2.5.1.47 1.36
-
 isoenzyme 1 Allium tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 additional information isoenzyme 1 and 2 have different substrate specificities towards various beta-substituted L-Cys Allium tuberosum ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + NaCN 6.84% and 7.64% of activity with sulfide, isoenzymes 1 and 2, respectively Allium tuberosum beta-cyanoalanine + sodium acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 40000, isoenzyme 1, SDS-PAGE Allium tuberosum
2.5.1.47 dimer 2 * 36000, isoenzyme 2, SDS-PAGE Allium tuberosum
2.5.1.47 More amino acid composition Allium tuberosum

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate one per subunit Allium tuberosum