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Literature summary extracted from

  • Yanase, H.; Sakai, T.; Tonomura, K.
    Some properties of a beta-cyanoalanine-forming enzyme of Enterobacter sp. 10-1 (1982), Agric. Biol. Chem., 46, 363-369.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 1,10-phenanthroline 14% inhibition at 1 mM Enterobacter sp.
2.5.1.47 Cd2+ 55% inhibition at 1 mM Enterobacter sp.
2.5.1.47 Cl- HgCl2 Enterobacter sp.
2.5.1.47 Co2+ complete inhibition at 1 mM Enterobacter sp.
2.5.1.47 hydroxylamine 31% inhibition at 1 mM Enterobacter sp.
2.5.1.47 Ni2+ complete inhibition at 1 mM Enterobacter sp.
2.5.1.47 p-chloromercuribenzoate 40% inhibition at 1 mM Enterobacter sp.
2.5.1.47 p-chloromercuriphenylsulfonic acid 46% inhibition at 1 mM Enterobacter sp.
2.5.1.47 Sodium borohydride 59% inhibition at 1 mM Enterobacter sp.
2.5.1.47 Thiourea 34% inhibition at 1 mM Enterobacter sp.
2.5.1.47 Zn2+ 95% inhibition at 1 mM Enterobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.5
-
H2S pH 7.4, 30°C Enterobacter sp.
2.5.1.47 5.2
-
NaCN pH 7.4, 30°C Enterobacter sp.
2.5.1.47 6.7 8.3 O-acetyl-L-Ser pH 7.4, 30°C, cosubstrate-dependent Enterobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Enterobacter sp.
-
-
-
2.5.1.47 Enterobacter sp. 10-1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + NaCN
-
Enterobacter sp. beta-cyanoalanine + sodium acetate
-
?
2.5.1.47 O-acetyl-L-Ser + NaCN
-
Enterobacter sp. 10-1 beta-cyanoalanine + sodium acetate
-
?
2.5.1.47 O-acetyl-L-serine
-
Enterobacter sp. L-cysteine + acetate
-
?
2.5.1.47 O-acetyl-L-serine
-
Enterobacter sp. 10-1 L-cysteine + acetate
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.47 50
-
10 min, unstable above Enterobacter sp.
2.5.1.47 60
-
10 min, no loss of activity Enterobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 9.75
-
-
Enterobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.47 8 10
-
Enterobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.47 6 10 37°C, 60 min, stable Enterobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate 0.75 mol per mol subunit Enterobacter sp.