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Literature summary extracted from

  • Yamagata, S.
    Occurrence of low molecular weight O-acetylserine sulfhydrylase in the yeast Saccharomyces cerevisiae (1980), J. Biochem., 88, 1419-1423.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 96000
-
 wild type strain, low molecular weight enzyme, sedimentation equilibrium Saccharomyces cerevisiae
2.5.1.47 99000
-
 Cys auxotroph strain, sedimentation equilibrium Saccharomyces cerevisiae
2.5.1.49 96000
-
 sucrose density gradient centrifugation , wild-type, O-acetylserine sulfhydrylase activity Saccharomyces cerevisiae
2.5.1.49 99000
-
 gel filtration, sucrose density gradient centrifugation , mutant strain No. 17, O-acetylserine sulfhydrylase activity Saccharomyces cerevisiae
2.5.1.49 182000
-
 gel filtration, mutant strain No. 17, sucrose density gradient centrifugation, O-acetylhomoserine sulfhydrylase activity Saccharomyces cerevisiae
2.5.1.49 194000
-
 sucrose density gradient centrifugation, wild-type, OAS-OAH sulfhydrylase with O-acetylserine as substrate Saccharomyces cerevisiae
2.5.1.49 196000
-
 sucrose density gradient centrifugation, wild-type, OAS-OAH sulfhydrylase with O-acetylhomoserine as substrate Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Saccharomyces cerevisiae
-
 low molecular weight enzyme, wild type and Cys auxotroph strain
-
2.5.1.49 Saccharomyces cerevisiae
-
 mutant strain No. 17
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Saccharomyces cerevisiae
2.5.1.49 mutant strain No. 17 Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 0.0283
-
 Cys-auxotroph strain, low molecular weight enzyme Saccharomyces cerevisiae
2.5.1.49 7.45
-
 mutant strain No. 17, O-acetylhomoserine sulfhydrylase activity Saccharomyces cerevisiae
2.5.1.49 28.3
-
 mutant strain No. 17, O-acetylserine sulfhydrylase activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetylhomoserine + H2S not, low molecular weight enzyme Saccharomyces cerevisiae homocysteine + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.49 OAS-OAH sulfhydrylase
-
 Saccharomyces cerevisiae