BRENDA - Enzyme Database

Substrate specificity and structure-activity relationships of gentamicin acetyltransferase I. The dependence of antibiotic resistance upon substrate Vmax/Km values

Williams, J.W.; Northrop, D.B.; J. Biol. Chem. 253, 5908-5914 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.60
2-deoxystreptamine
weak
Escherichia coli
2.3.1.60
amikacin
i.e. BB-K 8
Escherichia coli
2.3.1.60
garamine
-
Escherichia coli
2.3.1.60
Kanamine
-
Escherichia coli
2.3.1.60
kanamycin A
-
Escherichia coli
2.3.1.60
kanamycin C
-
Escherichia coli
2.3.1.60
lividomycin
-
Escherichia coli
2.3.1.60
Lividomycin B
-
Escherichia coli
2.3.1.60
additional information
inhibition kinetics
Escherichia coli
2.3.1.60
neomycin
best inhibitor
Escherichia coli
2.3.1.60
paromomycin
-
Escherichia coli
2.3.1.60
Paromomycin I
-
Escherichia coli
2.3.1.60
ribostamycin
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.60
additional information
-
additional information
kinetic pattern
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.60
Escherichia coli
-
containing R-factor JR88
-
2.3.1.60
Escherichia coli C600
-
containing R-factor JR88
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.60
acetyl-CoA + fortimicin A
-
637302
Escherichia coli
CoA + N2'-acetylfortimicin A
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin A
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin A
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin B
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin B1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B1
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin C1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
2.3.1.60
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
2.3.1.60
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
2.3.1.60
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
2.3.1.60
acetyl-CoA + gentamicin C2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C2
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin XK62-2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin XK62-2
-
-
-
?
2.3.1.60
acetyl-CoA + kanamycin B
-
637302
Escherichia coli
CoA + N3'-acetylkanamycin B
-
-
-
?
2.3.1.60
acetyl-CoA + N-ethylsisomicin
-
637302
Escherichia coli
CoA + N3'-acetylethylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + neamine
-
637302
Escherichia coli
CoA + N3'-acetylneamine
-
-
-
?
2.3.1.60
acetyl-CoA + nebramine
-
637302
Escherichia coli
CoA + N2'-acetylnebramine
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
637302
Escherichia coli
CoA + N3'-acetyltobramycin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
637302
Escherichia coli C600
CoA + N3'-acetyltobramycin
-
-
-
?
2.3.1.60
additional information
substrate structure/activity relationship
637302
Escherichia coli
?
-
-
-
-
2.3.1.60
additional information
substrate structure/activity relationship
637302
Escherichia coli C600
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.60
25
-
assay at
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.60
0.0004
-
neomycin
-
Escherichia coli
2.3.1.60
0.002
-
lividomycin
-
Escherichia coli
2.3.1.60
0.0036
-
garamine
-
Escherichia coli
2.3.1.60
0.0083
-
paromomycin
-
Escherichia coli
2.3.1.60
0.024
-
amikacin
-
Escherichia coli
2.3.1.60
0.026
-
kanamycin C
-
Escherichia coli
2.3.1.60
0.07
-
ribostamycin
-
Escherichia coli
2.3.1.60
0.093
-
kanamycin A
-
Escherichia coli
2.3.1.60
0.208
-
Kanamine
-
Escherichia coli
2.3.1.60
2
-
2-deoxystreptamine
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.60
2-deoxystreptamine
weak
Escherichia coli
2.3.1.60
amikacin
i.e. BB-K 8
Escherichia coli
2.3.1.60
garamine
-
Escherichia coli
2.3.1.60
Kanamine
-
Escherichia coli
2.3.1.60
kanamycin A
-
Escherichia coli
2.3.1.60
kanamycin C
-
Escherichia coli
2.3.1.60
lividomycin
-
Escherichia coli
2.3.1.60
Lividomycin B
-
Escherichia coli
2.3.1.60
additional information
inhibition kinetics
Escherichia coli
2.3.1.60
neomycin
best inhibitor
Escherichia coli
2.3.1.60
paromomycin
-
Escherichia coli
2.3.1.60
Paromomycin I
-
Escherichia coli
2.3.1.60
ribostamycin
-
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.60
0.0004
-
neomycin
-
Escherichia coli
2.3.1.60
0.002
-
lividomycin
-
Escherichia coli
2.3.1.60
0.0036
-
garamine
-
Escherichia coli
2.3.1.60
0.0083
-
paromomycin
-
Escherichia coli
2.3.1.60
0.024
-
amikacin
-
Escherichia coli
2.3.1.60
0.026
-
kanamycin C
-
Escherichia coli
2.3.1.60
0.07
-
ribostamycin
-
Escherichia coli
2.3.1.60
0.093
-
kanamycin A
-
Escherichia coli
2.3.1.60
0.208
-
Kanamine
-
Escherichia coli
2.3.1.60
2
-
2-deoxystreptamine
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.60
additional information
-
additional information
kinetic pattern
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.60
acetyl-CoA + fortimicin A
-
637302
Escherichia coli
CoA + N2'-acetylfortimicin A
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin A
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin A
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin B
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin B1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B1
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin C1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
2.3.1.60
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
2.3.1.60
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
2.3.1.60
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
2.3.1.60
acetyl-CoA + gentamicin C2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C2
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin XK62-2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin XK62-2
-
-
-
?
2.3.1.60
acetyl-CoA + kanamycin B
-
637302
Escherichia coli
CoA + N3'-acetylkanamycin B
-
-
-
?
2.3.1.60
acetyl-CoA + N-ethylsisomicin
-
637302
Escherichia coli
CoA + N3'-acetylethylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + neamine
-
637302
Escherichia coli
CoA + N3'-acetylneamine
-
-
-
?
2.3.1.60
acetyl-CoA + nebramine
-
637302
Escherichia coli
CoA + N2'-acetylnebramine
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
637302
Escherichia coli
CoA + N3'-acetyltobramycin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
637302
Escherichia coli C600
CoA + N3'-acetyltobramycin
-
-
-
?
2.3.1.60
additional information
substrate structure/activity relationship
637302
Escherichia coli
?
-
-
-
-
2.3.1.60
additional information
substrate structure/activity relationship
637302
Escherichia coli C600
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.60
25
-
assay at
Escherichia coli