Substrate specificity and structure-activity relationships of gentamicin acetyltransferase I. The dependence of antibiotic resistance upon substrate Vmax/Km values

Williams, J.W.; Northrop, D.B.; J. Biol. Chem. 253, 5908-5914 (1978)

Data extracted from this reference:

EC Number

Inhibitors

Commentary

Organism

Structure

2.3.1.60

2-deoxystreptamine

weak

Escherichia coli

2.3.1.60

amikacin

i.e. BB-K 8

Escherichia coli

2.3.1.60

garamine

-

Escherichia coli

2.3.1.60

Kanamine

-

Escherichia coli

2.3.1.60

kanamycin A

-

Escherichia coli

2.3.1.60

kanamycin C

-

Escherichia coli

2.3.1.60

lividomycin

-

Escherichia coli

2.3.1.60

Lividomycin B

-

Escherichia coli

2.3.1.60

additional information

inhibition kinetics

Escherichia coli

2.3.1.60

neomycin

best inhibitor

Escherichia coli

2.3.1.60

paromomycin

-

Escherichia coli

2.3.1.60

Paromomycin I

-

Escherichia coli

2.3.1.60

ribostamycin

-

Escherichia coli

EC Number

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2.3.1.60

acetyl-CoA + fortimicin A

-

637302

Escherichia coli

CoA + N2'-acetylfortimicin A

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin A

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin A

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin B

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin B

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin B1

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin B1

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin C1

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin C1

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin C1a

best substrate

637302

Escherichia coli

CoA + N3'-acetylgentamicin C1a

-

637302

Escherichia coli

?

2.3.1.60

acetyl-CoA + gentamicin C1a

enzyme modifies the 3-N-position of the deoxystreptamine ring

637302

Escherichia coli

CoA + N3'-acetylgentamicin C1a

-

637302

Escherichia coli

?

2.3.1.60

acetyl-CoA + gentamicin C1a

best substrate

637302

Escherichia coli C600

CoA + N3'-acetylgentamicin C1a

-

637302

Escherichia coli C600

?

2.3.1.60

acetyl-CoA + gentamicin C1a

enzyme modifies the 3-N-position of the deoxystreptamine ring

637302

Escherichia coli C600

CoA + N3'-acetylgentamicin C1a

-

637302

Escherichia coli C600

?

2.3.1.60

acetyl-CoA + gentamicin C2

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin C2

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin XK62-2

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin XK62-2

-

-

-

?

2.3.1.60

acetyl-CoA + kanamycin B

-

637302

Escherichia coli

CoA + N3'-acetylkanamycin B

-

-

-

?

2.3.1.60

acetyl-CoA + N-ethylsisomicin

-

637302

Escherichia coli

CoA + N3'-acetylethylsisomicin

-

-

-

?

2.3.1.60

acetyl-CoA + neamine

-

637302

Escherichia coli

CoA + N3'-acetylneamine

-

-

-

?

2.3.1.60

acetyl-CoA + nebramine

-

637302

Escherichia coli

CoA + N2'-acetylnebramine

-

-

-

?

2.3.1.60

acetyl-CoA + sisomicin

best substrate

637302

Escherichia coli

CoA + N3'-acetylsisomicin

-

-

-

?

2.3.1.60

acetyl-CoA + sisomicin

best substrate

637302

Escherichia coli C600

CoA + N3'-acetylsisomicin

-

-

-

?

2.3.1.60

acetyl-CoA + tobramycin

-

637302

Escherichia coli

CoA + N3'-acetyltobramycin

-

-

-

?

2.3.1.60

acetyl-CoA + tobramycin

-

637302

Escherichia coli C600

CoA + N3'-acetyltobramycin

-

-

-

?

2.3.1.60

additional information

substrate structure/activity relationship

637302

Escherichia coli

?

-

-

-

-

2.3.1.60

additional information

substrate structure/activity relationship

637302

Escherichia coli C600

?

-

-

-

-

EC Number

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

2.3.1.60

0.0004

-

neomycin

-

Escherichia coli

2.3.1.60

0.002

-

lividomycin

-

Escherichia coli

2.3.1.60

0.0036

-

garamine

-

Escherichia coli

2.3.1.60

0.0083

-

paromomycin

-

Escherichia coli

2.3.1.60

0.024

-

amikacin

-

Escherichia coli

2.3.1.60

0.026

-

kanamycin C

-

Escherichia coli

2.3.1.60

0.07

-

ribostamycin

-

Escherichia coli

2.3.1.60

0.093

-

kanamycin A

-

Escherichia coli

2.3.1.60

0.208

-

Kanamine

-

Escherichia coli

2.3.1.60

2

-

2-deoxystreptamine

-

Escherichia coli

EC Number

Inhibitors

Commentary

Organism

Structure

2.3.1.60

2-deoxystreptamine

weak

Escherichia coli

2.3.1.60

amikacin

i.e. BB-K 8

Escherichia coli

2.3.1.60

garamine

-

Escherichia coli

2.3.1.60

Kanamine

-

Escherichia coli

2.3.1.60

kanamycin A

-

Escherichia coli

2.3.1.60

kanamycin C

-

Escherichia coli

2.3.1.60

lividomycin

-

Escherichia coli

2.3.1.60

Lividomycin B

-

Escherichia coli

2.3.1.60

additional information

inhibition kinetics

Escherichia coli

2.3.1.60

neomycin

best inhibitor

Escherichia coli

2.3.1.60

paromomycin

-

Escherichia coli

2.3.1.60

Paromomycin I

-

Escherichia coli

2.3.1.60

ribostamycin

-

Escherichia coli

EC Number

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

2.3.1.60

0.0004

-

neomycin

-

Escherichia coli

2.3.1.60

0.002

-

lividomycin

-

Escherichia coli

2.3.1.60

0.0036

-

garamine

-

Escherichia coli

2.3.1.60

0.0083

-

paromomycin

-

Escherichia coli

2.3.1.60

0.024

-

amikacin

-

Escherichia coli

2.3.1.60

0.026

-

kanamycin C

-

Escherichia coli

2.3.1.60

0.07

-

ribostamycin

-

Escherichia coli

2.3.1.60

0.093

-

kanamycin A

-

Escherichia coli

2.3.1.60

0.208

-

Kanamine

-

Escherichia coli

2.3.1.60

2

-

2-deoxystreptamine

-

Escherichia coli

EC Number

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2.3.1.60

acetyl-CoA + fortimicin A

-

637302

Escherichia coli

CoA + N2'-acetylfortimicin A

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin A

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin A

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin B

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin B

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin B1

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin B1

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin C1

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin C1

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin C1a

best substrate

637302

Escherichia coli

CoA + N3'-acetylgentamicin C1a

-

637302

Escherichia coli

?

2.3.1.60

acetyl-CoA + gentamicin C1a

enzyme modifies the 3-N-position of the deoxystreptamine ring

637302

Escherichia coli

CoA + N3'-acetylgentamicin C1a

-

637302

Escherichia coli

?

2.3.1.60

acetyl-CoA + gentamicin C1a

best substrate

637302

Escherichia coli C600

CoA + N3'-acetylgentamicin C1a

-

637302

Escherichia coli C600

?

2.3.1.60

acetyl-CoA + gentamicin C1a

enzyme modifies the 3-N-position of the deoxystreptamine ring

637302

Escherichia coli C600

CoA + N3'-acetylgentamicin C1a

-

637302

Escherichia coli C600

?

2.3.1.60

acetyl-CoA + gentamicin C2

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin C2

-

-

-

?

2.3.1.60

acetyl-CoA + gentamicin XK62-2

-

637302

Escherichia coli

CoA + N3'-acetylgentamicin XK62-2

-

-

-

?

2.3.1.60

acetyl-CoA + kanamycin B

-

637302

Escherichia coli

CoA + N3'-acetylkanamycin B

-

-

-

?

2.3.1.60

acetyl-CoA + N-ethylsisomicin

-

637302

Escherichia coli

CoA + N3'-acetylethylsisomicin

-

-

-

?

2.3.1.60

acetyl-CoA + neamine

-

637302

Escherichia coli

CoA + N3'-acetylneamine

-

-

-

?

2.3.1.60

acetyl-CoA + nebramine

-

637302

Escherichia coli

CoA + N2'-acetylnebramine

-

-

-

?

2.3.1.60

acetyl-CoA + sisomicin

best substrate

637302

Escherichia coli

CoA + N3'-acetylsisomicin

-

-

-

?

2.3.1.60

acetyl-CoA + sisomicin

best substrate

637302

Escherichia coli C600

CoA + N3'-acetylsisomicin

-

-

-

?

2.3.1.60

acetyl-CoA + tobramycin

-

637302

Escherichia coli

CoA + N3'-acetyltobramycin

-

-

-

?

2.3.1.60

acetyl-CoA + tobramycin

-

637302

Escherichia coli C600

CoA + N3'-acetyltobramycin

-

-

-

?

2.3.1.60

additional information

substrate structure/activity relationship

637302

Escherichia coli

?

-

-

-

-

2.3.1.60

additional information

substrate structure/activity relationship

637302

Escherichia coli C600

?

-

-

-

-