BRENDA - Enzyme Database

Substrate specificity and structure-activity relationships of gentamicin acetyltransferase I. The dependence of antibiotic resistance upon substrate Vmax/Km values

Williams, J.W.; Northrop, D.B.; J. Biol. Chem. 253, 5908-5914 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.60
2-deoxystreptamine
weak
Escherichia coli
2.3.1.60
amikacin
i.e. BB-K 8
Escherichia coli
2.3.1.60
garamine
-
Escherichia coli
2.3.1.60
Kanamine
-
Escherichia coli
2.3.1.60
kanamycin A
-
Escherichia coli
2.3.1.60
kanamycin C
-
Escherichia coli
2.3.1.60
lividomycin
-
Escherichia coli
2.3.1.60
Lividomycin B
-
Escherichia coli
2.3.1.60
additional information
inhibition kinetics
Escherichia coli
2.3.1.60
neomycin
best inhibitor
Escherichia coli
2.3.1.60
paromomycin
-
Escherichia coli
2.3.1.60
Paromomycin I
-
Escherichia coli
2.3.1.60
ribostamycin
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.60
additional information
-
additional information
kinetic pattern
Escherichia coli
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.60
Escherichia coli
-
containing R-factor JR88
-
2.3.1.60
Escherichia coli C600
-
containing R-factor JR88
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.60
acetyl-CoA + fortimicin A
-
637302
Escherichia coli
CoA + N2'-acetylfortimicin A
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin A
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin A
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin B
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin B1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B1
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin C1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
2.3.1.60
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
2.3.1.60
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
2.3.1.60
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
2.3.1.60
acetyl-CoA + gentamicin C2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C2
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin XK62-2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin XK62-2
-
-
-
?
2.3.1.60
acetyl-CoA + kanamycin B
-
637302
Escherichia coli
CoA + N3'-acetylkanamycin B
-
-
-
?
2.3.1.60
acetyl-CoA + N-ethylsisomicin
-
637302
Escherichia coli
CoA + N3'-acetylethylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + neamine
-
637302
Escherichia coli
CoA + N3'-acetylneamine
-
-
-
?
2.3.1.60
acetyl-CoA + nebramine
-
637302
Escherichia coli
CoA + N2'-acetylnebramine
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
637302
Escherichia coli
CoA + N3'-acetyltobramycin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
637302
Escherichia coli C600
CoA + N3'-acetyltobramycin
-
-
-
?
2.3.1.60
additional information
substrate structure/activity relationship
637302
Escherichia coli
?
-
-
-
-
2.3.1.60
additional information
substrate structure/activity relationship
637302
Escherichia coli C600
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.60
25
-
assay at
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.60
0.0004
-
neomycin
-
Escherichia coli
2.3.1.60
0.002
-
lividomycin
-
Escherichia coli
2.3.1.60
0.0036
-
garamine
-
Escherichia coli
2.3.1.60
0.0083
-
paromomycin
-
Escherichia coli
2.3.1.60
0.024
-
amikacin
-
Escherichia coli
2.3.1.60
0.026
-
kanamycin C
-
Escherichia coli
2.3.1.60
0.07
-
ribostamycin
-
Escherichia coli
2.3.1.60
0.093
-
kanamycin A
-
Escherichia coli
2.3.1.60
0.208
-
Kanamine
-
Escherichia coli
2.3.1.60
2
-
2-deoxystreptamine
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.60
2-deoxystreptamine
weak
Escherichia coli
2.3.1.60
amikacin
i.e. BB-K 8
Escherichia coli
2.3.1.60
garamine
-
Escherichia coli
2.3.1.60
Kanamine
-
Escherichia coli
2.3.1.60
kanamycin A
-
Escherichia coli
2.3.1.60
kanamycin C
-
Escherichia coli
2.3.1.60
lividomycin
-
Escherichia coli
2.3.1.60
Lividomycin B
-
Escherichia coli
2.3.1.60
additional information
inhibition kinetics
Escherichia coli
2.3.1.60
neomycin
best inhibitor
Escherichia coli
2.3.1.60
paromomycin
-
Escherichia coli
2.3.1.60
Paromomycin I
-
Escherichia coli
2.3.1.60
ribostamycin
-
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.60
0.0004
-
neomycin
-
Escherichia coli
2.3.1.60
0.002
-
lividomycin
-
Escherichia coli
2.3.1.60
0.0036
-
garamine
-
Escherichia coli
2.3.1.60
0.0083
-
paromomycin
-
Escherichia coli
2.3.1.60
0.024
-
amikacin
-
Escherichia coli
2.3.1.60
0.026
-
kanamycin C
-
Escherichia coli
2.3.1.60
0.07
-
ribostamycin
-
Escherichia coli
2.3.1.60
0.093
-
kanamycin A
-
Escherichia coli
2.3.1.60
0.208
-
Kanamine
-
Escherichia coli
2.3.1.60
2
-
2-deoxystreptamine
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.60
additional information
-
additional information
kinetic pattern
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.60
acetyl-CoA + fortimicin A
-
637302
Escherichia coli
CoA + N2'-acetylfortimicin A
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin A
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin A
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin B
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin B1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B1
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin C1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
2.3.1.60
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
2.3.1.60
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
2.3.1.60
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
2.3.1.60
acetyl-CoA + gentamicin C2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C2
-
-
-
?
2.3.1.60
acetyl-CoA + gentamicin XK62-2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin XK62-2
-
-
-
?
2.3.1.60
acetyl-CoA + kanamycin B
-
637302
Escherichia coli
CoA + N3'-acetylkanamycin B
-
-
-
?
2.3.1.60
acetyl-CoA + N-ethylsisomicin
-
637302
Escherichia coli
CoA + N3'-acetylethylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + neamine
-
637302
Escherichia coli
CoA + N3'-acetylneamine
-
-
-
?
2.3.1.60
acetyl-CoA + nebramine
-
637302
Escherichia coli
CoA + N2'-acetylnebramine
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
637302
Escherichia coli
CoA + N3'-acetyltobramycin
-
-
-
?
2.3.1.60
acetyl-CoA + tobramycin
-
637302
Escherichia coli C600
CoA + N3'-acetyltobramycin
-
-
-
?
2.3.1.60
additional information
substrate structure/activity relationship
637302
Escherichia coli
?
-
-
-
-
2.3.1.60
additional information
substrate structure/activity relationship
637302
Escherichia coli C600
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.60
25
-
assay at
Escherichia coli