BRENDA - Enzyme Database

Kinetic mechanisms of gentamicin acetyltransferase I. Antibiotic-dependent shift from rapid to nonrapid equilibrium random mechanisms

Williams, J.W.; Northrop, D.B.; J. Biol. Chem. 253, 5902-5907 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.81
acetyltobramycin
-
Escherichia coli
2.3.1.81
amikacin
-
Escherichia coli
2.3.1.81
butyryl-CoA
dead-end inhibition pattern
Escherichia coli
2.3.1.81
CoA
product inhibition
Escherichia coli
2.3.1.81
gentamicin C1a
substrate inhibition
Escherichia coli
2.3.1.81
additional information
inhibition kinetics
Escherichia coli
2.3.1.81
N3-Acetyltobramycin
product inhibition
Escherichia coli
2.3.1.81
neomycin
-
Escherichia coli
2.3.1.81
ribostamycin
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.81
additional information
-
additional information
kinetic pattern
Escherichia coli
2.3.1.81
0.00012
-
gentamicin C1a
-
Escherichia coli
2.3.1.81
0.0013
-
acetyl-CoA
cosubstrate gentamicin C1a
Escherichia coli
2.3.1.81
0.0016
-
tobramycin
-
Escherichia coli
2.3.1.81
0.0017
-
acetyl-CoA
cosubstrate tobramycin
Escherichia coli
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.81
Escherichia coli
-
containing R-factor JR88
-
2.3.1.81
Escherichia coli C600
-
containing R-factor JR88
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.81
acetyl-CoA + gentamicin C1a
-
637301
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637301
Escherichia coli
?
2.3.1.81
acetyl-CoA + gentamicin C1a
-
637301
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637301
Escherichia coli C600
?
2.3.1.81
acetyl-CoA + sisomicin
-
637301
Escherichia coli
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.81
acetyl-CoA + sisomicin
-
637301
Escherichia coli C600
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.81
acetyl-CoA + tobramycin
-
637301
Escherichia coli
CoA + N3'-acetyltobramycin
-
-
-
?
2.3.1.81
acetyl-CoA + tobramycin
-
637301
Escherichia coli C600
CoA + N3'-acetyltobramycin
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.81
25
-
assay at
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.81
acetyltobramycin
-
Escherichia coli
2.3.1.81
amikacin
-
Escherichia coli
2.3.1.81
butyryl-CoA
dead-end inhibition pattern
Escherichia coli
2.3.1.81
CoA
product inhibition
Escherichia coli
2.3.1.81
gentamicin C1a
substrate inhibition
Escherichia coli
2.3.1.81
additional information
inhibition kinetics
Escherichia coli
2.3.1.81
N3-Acetyltobramycin
product inhibition
Escherichia coli
2.3.1.81
neomycin
-
Escherichia coli
2.3.1.81
ribostamycin
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.81
additional information
-
additional information
kinetic pattern
Escherichia coli
2.3.1.81
0.00012
-
gentamicin C1a
-
Escherichia coli
2.3.1.81
0.0013
-
acetyl-CoA
cosubstrate gentamicin C1a
Escherichia coli
2.3.1.81
0.0016
-
tobramycin
-
Escherichia coli
2.3.1.81
0.0017
-
acetyl-CoA
cosubstrate tobramycin
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.81
acetyl-CoA + gentamicin C1a
-
637301
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637301
Escherichia coli
?
2.3.1.81
acetyl-CoA + gentamicin C1a
-
637301
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637301
Escherichia coli C600
?
2.3.1.81
acetyl-CoA + sisomicin
-
637301
Escherichia coli
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.81
acetyl-CoA + sisomicin
-
637301
Escherichia coli C600
CoA + N3'-acetylsisomicin
-
-
-
?
2.3.1.81
acetyl-CoA + tobramycin
-
637301
Escherichia coli
CoA + N3'-acetyltobramycin
-
-
-
?
2.3.1.81
acetyl-CoA + tobramycin
-
637301
Escherichia coli C600
CoA + N3'-acetyltobramycin
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.81
25
-
assay at
Escherichia coli