BRENDA - Enzyme Database

Purification and properties of gentamicin acetyltransferase I

Williams, J.W.; Northrop, D.B.; Biochemistry 15, 125-131 (1976)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.60
additional information
N-methylmaleimide and DTT have no effect on activity
Escherichia coli
General Stability
EC Number
General Stability
Organism
2.3.1.60
lyophilization, stable to
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.60
gentamicin
-
Escherichia coli
2.3.1.60
gentamicin C1a
substrate inhibition
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.60
0.0003
0.0005
gentamicin C1a
-
Escherichia coli
2.3.1.60
0.0039
0.0048
acetyl-CoA
-
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.60
cytoplasm
-
Escherichia coli
5737
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.60
17000
-
4 * 17000, SDS-PAGE
Escherichia coli
2.3.1.60
63000
-
equilibrium sedimentation centrifugation
Escherichia coli
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.3.1.60
Escherichia coli
-
containing R-factor JR88
-
2.3.1.60
Escherichia coli C600
-
containing R-factor JR88
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.3.1.60
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.60
3.8
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.60
20°C, lyophilized, up to 1 month
Escherichia coli
2.3.1.60
frozen, redissolved ammonium sulfate precipitate, at least 1 month
Escherichia coli
2.3.1.60
refrigerated, lyophilized, up to 1 month
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.3.1.60
acetyl-CoA + gentamicin C1a
-
637298
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637298
Escherichia coli
?
2.3.1.60
acetyl-CoA + gentamicin C1a
-
637298
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637298
Escherichia coli C600
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.60
tetramer
4 * 17000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.60
25
-
assay at
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.60
3.57
-
N3'-acetylgentamicin C1a
-
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.60
7.7
8
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.60
5.5
8.5
-
Escherichia coli
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.60
4
-
irreversible inactivation
Escherichia coli
2.3.1.60
4.5
-
no activity, restorable by neutralization
Escherichia coli
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.60
0.16
-
gentamicin
-
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.60
additional information
N-methylmaleimide and DTT have no effect on activity
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.60
lyophilization, stable to
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.60
gentamicin
-
Escherichia coli
2.3.1.60
gentamicin C1a
substrate inhibition
Escherichia coli
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.3.1.60
0.16
-
gentamicin
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.60
0.0003
0.0005
gentamicin C1a
-
Escherichia coli
2.3.1.60
0.0039
0.0048
acetyl-CoA
-
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.60
cytoplasm
-
Escherichia coli
5737
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.60
17000
-
4 * 17000, SDS-PAGE
Escherichia coli
2.3.1.60
63000
-
equilibrium sedimentation centrifugation
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.60
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.60
3.8
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.60
20°C, lyophilized, up to 1 month
Escherichia coli
2.3.1.60
frozen, redissolved ammonium sulfate precipitate, at least 1 month
Escherichia coli
2.3.1.60
refrigerated, lyophilized, up to 1 month
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.3.1.60
acetyl-CoA + gentamicin C1a
-
637298
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637298
Escherichia coli
?
2.3.1.60
acetyl-CoA + gentamicin C1a
-
637298
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637298
Escherichia coli C600
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.60
tetramer
4 * 17000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.60
25
-
assay at
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.60
3.57
-
N3'-acetylgentamicin C1a
-
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.60
7.7
8
-
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.60
5.5
8.5
-
Escherichia coli
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.60
4
-
irreversible inactivation
Escherichia coli
2.3.1.60
4.5
-
no activity, restorable by neutralization
Escherichia coli