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Literature summary extracted from

  • Mathews, I.I.; Deacon, A.M.; Canaves, J.M.; McMullan, D.; Lesley, S.A.; Agarwalla, S.; Kuhn, P.
    Functional analysis of substrate and cofactor complex structures of a thymidylate synthase-complementing protein (2003), Structure, 11, 677-690.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.148 NADH
-
 Helicobacter pylori
2.1.1.148 NADH required to reduce bound FAD Dictyostelium discoideum
2.1.1.148 NADH required to reduce bound FAD Thermotoga maritima
2.1.1.148 NADPH
-
 Helicobacter pylori
2.1.1.148 NADPH required to reduce bound FAD Dictyostelium discoideum
2.1.1.148 NADPH required to reduce bound FAD Thermotoga maritima

Application

EC Number Application Comment Organism
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Chlamydia trachomatis
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Mycobacterium avium
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Rhodococcus sp.
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Clostridium perfringens
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Mycobacterium tuberculosis variant bovis
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Clostridium botulinum
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Clostridioides difficile
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Corynebacterium diphtheriae
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Treponema denticola
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Treponema pallidum
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Rickettsia prowazekii
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Borreliella burgdorferi
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Campylobacter jejuni
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Chlamydia pneumoniae
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Chlamydia psittaci
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Helicobacter pylori
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Bacillus anthracis
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Rickettsia conorii
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Ehrlichia chaffeensis
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Chlamydia trachomatis
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Mycobacterium avium
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Mycobacterium leprae
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Mycobacterium tuberculosis
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Helicobacter pylori
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Rhodococcus sp.
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Clostridium perfringens
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Mycobacterium tuberculosis variant bovis
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Treponema denticola
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Treponema pallidum
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Rickettsia prowazekii
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Borreliella burgdorferi
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Campylobacter jejuni
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Chlamydia pneumoniae
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Chlamydia psittaci
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Bacillus anthracis
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Rickettsia conorii
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low cross-reactivity against mammalian thyA enzymes, EC 2.1.1.45 Ehrlichia chaffeensis
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low crossreactivity against mammalian thyA enzymes, EC 2.1.1.45 Clostridium botulinum
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low crossreactivity against mammalian thyA enzymes, EC 2.1.1.45 Clostridioides difficile
2.1.1.148 medicine specific and selective inhibitors for thy1 can provide highly effective tools for therapeutic intervention with low crossreactivity against mammalian thyA enzymes, EC 2.1.1.45 Corynebacterium diphtheriae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.148
-
 Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.148
-
 Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.148 FAD
-
 Thermotoga maritima
2.1.1.148 HEPES
-
 Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Chlamydia trachomatis
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Dictyostelium discoideum
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Mycobacterium avium
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Mycobacterium leprae
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Mycobacterium tuberculosis
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Rhodococcus sp.
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Clostridium perfringens
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Mycobacterium tuberculosis variant bovis
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Clostridium botulinum
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Clostridioides difficile
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Corynebacterium diphtheriae
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Treponema denticola
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Treponema pallidum
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Rickettsia prowazekii
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Borreliella burgdorferi
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Campylobacter jejuni
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Chlamydia pneumoniae
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Chlamydia psittaci
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Helicobacter pylori
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Thermotoga maritima
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Bacillus anthracis
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Rickettsia conorii
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Ehrlichia chaffeensis
-
 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Thermotoga maritima DSM 3109
-
 dTMP + tetrahydrofolate + FAD
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.148 Bacillus anthracis
-
-
-
2.1.1.148 Borreliella burgdorferi
-
-
-
2.1.1.148 Campylobacter jejuni
-
-
-
2.1.1.148 Chlamydia pneumoniae
-
-
-
2.1.1.148 Chlamydia psittaci
-
-
-
2.1.1.148 Chlamydia trachomatis
-
-
-
2.1.1.148 Clostridioides difficile
-
-
-
2.1.1.148 Clostridium botulinum
-
-
-
2.1.1.148 Clostridium perfringens
-
-
-
2.1.1.148 Corynebacterium diphtheriae
-
-
-
2.1.1.148 Dictyostelium discoideum
-
-
-
2.1.1.148 Ehrlichia chaffeensis
-
-
-
2.1.1.148 Helicobacter pylori
-
-
-
2.1.1.148 Helicobacter pylori Q9ZJ93
-
-
2.1.1.148 Mycobacterium avium
-
-
-
2.1.1.148 Mycobacterium leprae
-
-
-
2.1.1.148 Mycobacterium leprae
-
 simultaneous occurrence of thyA and thyX
-
2.1.1.148 Mycobacterium tuberculosis
-
-
-
2.1.1.148 Mycobacterium tuberculosis
-
 simultaneous occurrence of thyA and thyX
-
2.1.1.148 Mycobacterium tuberculosis variant bovis
-
-
-
2.1.1.148 Rhodococcus sp.
-
-
-
2.1.1.148 Rickettsia conorii
-
-
-
2.1.1.148 Rickettsia prowazekii
-
-
-
2.1.1.148 Thermotoga maritima Q9WYT0
-
-
2.1.1.148 Thermotoga maritima Q9WYT0 TM0449
-
2.1.1.148 Thermotoga maritima DSM 3109 Q9WYT0 TM0449
-
2.1.1.148 Treponema denticola
-
-
-
2.1.1.148 Treponema pallidum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.148
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Chlamydia trachomatis dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Dictyostelium discoideum dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Mycobacterium avium dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Mycobacterium leprae dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Mycobacterium tuberculosis dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Helicobacter pylori dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Rhodococcus sp. dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Clostridium perfringens dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Mycobacterium tuberculosis variant bovis dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Clostridium botulinum dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Clostridioides difficile dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Corynebacterium diphtheriae dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Treponema denticola dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Treponema pallidum dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Rickettsia prowazekii dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Borreliella burgdorferi dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Campylobacter jejuni dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Chlamydia pneumoniae dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Chlamydia psittaci dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Thermotoga maritima dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Bacillus anthracis dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Rickettsia conorii dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Ehrlichia chaffeensis dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Thermotoga maritima DSM 3109 dTMP + tetrahydrofolate + FAD
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.148 tetramer
-
 Helicobacter pylori
2.1.1.148 tetramer data from crystal structure Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.1.1.148 complementing thymidylate synthase
-
 Chlamydia trachomatis
2.1.1.148 complementing thymidylate synthase
-
 Mycobacterium avium
2.1.1.148 complementing thymidylate synthase
-
 Mycobacterium leprae
2.1.1.148 complementing thymidylate synthase
-
 Mycobacterium tuberculosis
2.1.1.148 complementing thymidylate synthase
-
 Helicobacter pylori
2.1.1.148 complementing thymidylate synthase
-
 Rhodococcus sp.
2.1.1.148 complementing thymidylate synthase
-
 Clostridium perfringens
2.1.1.148 complementing thymidylate synthase
-
 Mycobacterium tuberculosis variant bovis
2.1.1.148 complementing thymidylate synthase
-
 Clostridium botulinum
2.1.1.148 complementing thymidylate synthase
-
 Clostridioides difficile
2.1.1.148 complementing thymidylate synthase
-
 Corynebacterium diphtheriae
2.1.1.148 complementing thymidylate synthase
-
 Treponema denticola
2.1.1.148 complementing thymidylate synthase
-
 Treponema pallidum
2.1.1.148 complementing thymidylate synthase
-
 Rickettsia prowazekii
2.1.1.148 complementing thymidylate synthase
-
 Borreliella burgdorferi
2.1.1.148 complementing thymidylate synthase
-
 Campylobacter jejuni
2.1.1.148 complementing thymidylate synthase
-
 Chlamydia pneumoniae
2.1.1.148 complementing thymidylate synthase
-
 Chlamydia psittaci
2.1.1.148 complementing thymidylate synthase
-
 Thermotoga maritima
2.1.1.148 complementing thymidylate synthase
-
 Bacillus anthracis
2.1.1.148 complementing thymidylate synthase
-
 Rickettsia conorii
2.1.1.148 complementing thymidylate synthase
-
 Ehrlichia chaffeensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.148 FAD
-
 Chlamydia trachomatis
2.1.1.148 FAD
-
 Mycobacterium avium
2.1.1.148 FAD
-
 Mycobacterium leprae
2.1.1.148 FAD
-
 Mycobacterium tuberculosis
2.1.1.148 FAD
-
 Helicobacter pylori
2.1.1.148 FAD
-
 Rhodococcus sp.
2.1.1.148 FAD
-
 Clostridium perfringens
2.1.1.148 FAD
-
 Mycobacterium tuberculosis variant bovis
2.1.1.148 FAD
-
 Clostridium botulinum
2.1.1.148 FAD
-
 Clostridioides difficile
2.1.1.148 FAD
-
 Corynebacterium diphtheriae
2.1.1.148 FAD
-
 Treponema denticola
2.1.1.148 FAD
-
 Treponema pallidum
2.1.1.148 FAD
-
 Rickettsia prowazekii
2.1.1.148 FAD
-
 Borreliella burgdorferi
2.1.1.148 FAD
-
 Campylobacter jejuni
2.1.1.148 FAD
-
 Chlamydia pneumoniae
2.1.1.148 FAD
-
 Chlamydia psittaci
2.1.1.148 FAD
-
 Bacillus anthracis
2.1.1.148 FAD
-
 Rickettsia conorii
2.1.1.148 FAD
-
 Ehrlichia chaffeensis
2.1.1.148 FAD tightly bound, four molecules per tetramer Dictyostelium discoideum
2.1.1.148 FAD tightly bound, four molecules per tetramer Thermotoga maritima
2.1.1.148 FAD even though FAD is very well bound to three subunits, FAD interactions are not an absolute requirement for tetramer stabilization Thermotoga maritima
2.1.1.148 FADH2
-
 Chlamydia trachomatis
2.1.1.148 FADH2
-
 Mycobacterium avium
2.1.1.148 FADH2
-
 Mycobacterium leprae
2.1.1.148 FADH2
-
 Mycobacterium tuberculosis
2.1.1.148 FADH2
-
 Helicobacter pylori
2.1.1.148 FADH2
-
 Rhodococcus sp.
2.1.1.148 FADH2
-
 Clostridium perfringens
2.1.1.148 FADH2
-
 Mycobacterium tuberculosis variant bovis
2.1.1.148 FADH2
-
 Clostridium botulinum
2.1.1.148 FADH2
-
 Clostridioides difficile
2.1.1.148 FADH2
-
 Corynebacterium diphtheriae
2.1.1.148 FADH2
-
 Treponema denticola
2.1.1.148 FADH2
-
 Treponema pallidum
2.1.1.148 FADH2
-
 Rickettsia prowazekii
2.1.1.148 FADH2
-
 Borreliella burgdorferi
2.1.1.148 FADH2
-
 Campylobacter jejuni
2.1.1.148 FADH2
-
 Chlamydia pneumoniae
2.1.1.148 FADH2
-
 Chlamydia psittaci
2.1.1.148 FADH2
-
 Thermotoga maritima
2.1.1.148 FADH2
-
 Bacillus anthracis
2.1.1.148 FADH2
-
 Rickettsia conorii
2.1.1.148 FADH2
-
 Ehrlichia chaffeensis
2.1.1.148 NAD(P)H required Thermotoga maritima