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Literature summary extracted from

  • Myllykallio, H.; Lipowski, G.; Leduc, D.; Filee, J.; Forterre, P.; Liebl, U.
    An alternative flavin-dependent mechanism for thymidylate synthesis (2002), Science, 297, 105-107.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.148 FAD increases activity Helicobacter pylori
2.1.1.148 FMN increases activity Helicobacter pylori
2.1.1.148 UMP
-
 Helicobacter pylori

Application

EC Number Application Comment Organism
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Rickettsia prowazekii
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Borreliella burgdorferi
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Campylobacter jejuni
2.1.1.148 medicine promising medical target, thyX is present in a number of pathogenic bacteria but absent in human Helicobacter pylori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.148 complemetation of Escherichia coli, transformants are able to grow in the absence of thymidine Helicobacter pylori
2.1.1.148 complemetation of Escherichia coli, transformants are unable to grow in the absence of thymidine Pyrococcus abyssi

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.148 S107A no activity Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.148 31000
-
 SDS-PAGE Helicobacter pylori
2.1.1.148 31500
-
 calculated from DNA sequence Helicobacter pylori
2.1.1.148 111000
-
 gel filtration Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Dictyostelium discoideum de novo synthesis of thymidylate dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Mycobacterium tuberculosis de novo synthesis of thymidylate dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Saccharolobus solfataricus de novo synthesis of thymidylate dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Pyrococcus horikoshii de novo synthesis of thymidylate dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Treponema pallidum de novo synthesis of thymidylate dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Rickettsia prowazekii de novo synthesis of thymidylate dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Borreliella burgdorferi de novo synthesis of thymidylate dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Chlamydia sp. de novo synthesis of thymidylate dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Campylobacter jejuni de novo synthesis of thymidylate dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Helicobacter pylori de novo synthesis of thymidylate dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 Pyrococcus abyssi de novo synthesis of thymidylate dTMP + tetrahydrofolate + FAD
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.148 Borreliella burgdorferi
-
-
-
2.1.1.148 Campylobacter jejuni
-
-
-
2.1.1.148 Chlamydia sp.
-
 enzyme found in three different species
-
2.1.1.148 Dictyostelium discoideum
-
-
-
2.1.1.148 Helicobacter pylori Q9ZJ93
-
-
2.1.1.148 Mycobacterium tuberculosis
-
-
-
2.1.1.148 Pyrococcus abyssi Q9UZ51
-
-
2.1.1.148 Pyrococcus horikoshii
-
-
-
2.1.1.148 Rickettsia prowazekii
-
-
-
2.1.1.148 Saccharolobus solfataricus
-
-
-
2.1.1.148 Treponema pallidum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.148 from transformed E. coli using His-tag Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Dictyostelium discoideum dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Mycobacterium tuberculosis dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Saccharolobus solfataricus dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Pyrococcus horikoshii dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Treponema pallidum dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Rickettsia prowazekii dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Borreliella burgdorferi dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Chlamydia sp. dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Campylobacter jejuni dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Helicobacter pylori dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Pyrococcus abyssi dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 de novo synthesis of thymidylate Dictyostelium discoideum dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 de novo synthesis of thymidylate Mycobacterium tuberculosis dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 de novo synthesis of thymidylate Saccharolobus solfataricus dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 de novo synthesis of thymidylate Pyrococcus horikoshii dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 de novo synthesis of thymidylate Treponema pallidum dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 de novo synthesis of thymidylate Rickettsia prowazekii dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 de novo synthesis of thymidylate Borreliella burgdorferi dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 de novo synthesis of thymidylate Chlamydia sp. dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 de novo synthesis of thymidylate Campylobacter jejuni dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 de novo synthesis of thymidylate Helicobacter pylori dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2 de novo synthesis of thymidylate Pyrococcus abyssi dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FMNH2
-
 Dictyostelium discoideum dTMP + tetrahydrofolate + FMN
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FMNH2
-
 Mycobacterium tuberculosis dTMP + tetrahydrofolate + FMN
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FMNH2
-
 Saccharolobus solfataricus dTMP + tetrahydrofolate + FMN
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FMNH2
-
 Pyrococcus horikoshii dTMP + tetrahydrofolate + FMN
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FMNH2
-
 Treponema pallidum dTMP + tetrahydrofolate + FMN
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FMNH2
-
 Rickettsia prowazekii dTMP + tetrahydrofolate + FMN
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FMNH2
-
 Borreliella burgdorferi dTMP + tetrahydrofolate + FMN
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FMNH2
-
 Chlamydia sp. dTMP + tetrahydrofolate + FMN
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FMNH2
-
 Campylobacter jejuni dTMP + tetrahydrofolate + FMN
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FMNH2
-
 Helicobacter pylori dTMP + tetrahydrofolate + FMN
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FMNH2
-
 Pyrococcus abyssi dTMP + tetrahydrofolate + FMN
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.148 tetramer 4 * 31000, gel filtration, SDS-PAGE Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.148 FADH2
-
 Dictyostelium discoideum
2.1.1.148 FADH2
-
 Mycobacterium tuberculosis
2.1.1.148 FADH2
-
 Saccharolobus solfataricus
2.1.1.148 FADH2
-
 Pyrococcus horikoshii
2.1.1.148 FADH2
-
 Treponema pallidum
2.1.1.148 FADH2
-
 Rickettsia prowazekii
2.1.1.148 FADH2
-
 Borreliella burgdorferi
2.1.1.148 FADH2
-
 Chlamydia sp.
2.1.1.148 FADH2
-
 Campylobacter jejuni
2.1.1.148 FADH2
-
 Pyrococcus abyssi
2.1.1.148 FADH2 activity is dependent on reduced flavin nucleotides Helicobacter pylori
2.1.1.148 FMNH2
-
 Dictyostelium discoideum
2.1.1.148 FMNH2
-
 Mycobacterium tuberculosis
2.1.1.148 FMNH2
-
 Saccharolobus solfataricus
2.1.1.148 FMNH2
-
 Pyrococcus horikoshii
2.1.1.148 FMNH2
-
 Treponema pallidum
2.1.1.148 FMNH2
-
 Rickettsia prowazekii
2.1.1.148 FMNH2
-
 Borreliella burgdorferi
2.1.1.148 FMNH2
-
 Chlamydia sp.
2.1.1.148 FMNH2
-
 Campylobacter jejuni
2.1.1.148 FMNH2
-
 Pyrococcus abyssi
2.1.1.148 FMNH2 activity is dependent on reduced flavin nucleotides Helicobacter pylori