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Literature summary extracted from

  • Schwartz, N.B.; Rodén, L.
    Biosynthesis of chondroitin sulfate. Solubilization of chondroitin sulfate glycosyltransferases and partial purification of uridine diphosphate-D-galactose:D-xylose galactosyltrans (1975), J. Biol. Chem., 250, 5200-5207.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.133 0.05
-
 UDPgalactose pH 6.5, 30°C Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.133 membrane
-
 Gallus gallus 16020
-
2.4.1.134 membrane
-
 Gallus gallus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.133 Mn2+ requirement, 15 mM Gallus gallus
2.4.1.134 Mn2+ requirement Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.133 UDPgalactose + O-beta-D-xylosylprotein Gallus gallus involved in chondroitin sulfate biosynthesis UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
-
 ?
2.4.1.134 UDP-galactose + 4-beta-D-galactosyl-O-beta-D-xylosylprotein Gallus gallus involved in biosynthesis of chondroitin sulfate UDP + beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosylprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.133 Gallus gallus
-
-
-
2.4.1.134 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.133 solubilized and partially purified, affinity chromatography Gallus gallus
2.4.1.134 partial Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.133 cartilage epiphyses from embryonic femurs and tibiae Gallus gallus
-
2.4.1.134 cartilage epiphyses from embryonic femurs and tibiae Gallus gallus
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.133 -17°C, concentrated enzyme preparation of 1 mg/ml, at least 6 months Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.133 UDP-alpha-D-galactose + D-xylose
-
 Gallus gallus UDP + 4-O-beta-D-galactosyl-beta-D-xylose
-
 ?
2.4.1.133 UDPgalactose + O-beta-D-xylosylprotein involved in chondroitin sulfate biosynthesis Gallus gallus UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
-
 ?
2.4.1.134 UDP-galactose + 4-beta-D-galactosyl-O-beta-D-xylose
-
 Gallus gallus UDP + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylose
-
 ?
2.4.1.134 UDP-galactose + 4-beta-D-galactosyl-O-beta-D-xylosylprotein involved in biosynthesis of chondroitin sulfate Gallus gallus UDP + beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosylprotein
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.133 37
-
 assay at Gallus gallus
2.4.1.134 37
-
 assay at Gallus gallus