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Literature summary extracted from

  • Kalita, C.; Kerman, J.D.; Strecker, H.J.
    Preparation and properties of ornithine-oxo-acid aminotransferase of rat kidney. Comparison with the liver enzyme (1976), Biochim. Biophys. Acta, 429, 780-797.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.13
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.13 4-aminobutanoate
-
 Rattus norvegicus
2.6.1.13 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Rattus norvegicus
2.6.1.13 5-Aminopentanoate
-
 Rattus norvegicus
2.6.1.13 Butanoate
-
 Rattus norvegicus
2.6.1.13 Butyrate 27% inhibition at 25 mM Rattus norvegicus
2.6.1.13 caproate 34% inhibition at 25 mM Rattus norvegicus
2.6.1.13 DL-norvaline 44% inhibition at 100 mM Rattus norvegicus
2.6.1.13 hexanoate
-
 Rattus norvegicus
2.6.1.13 iodoacetamide
-
 Rattus norvegicus
2.6.1.13 Isocaproate 41% inhibition at 25 mM Rattus norvegicus
2.6.1.13 Isohexanoate
-
 Rattus norvegicus
2.6.1.13 Isopentanoate
-
 Rattus norvegicus
2.6.1.13 Isovalerate 41% inhibition at 25 mM Rattus norvegicus
2.6.1.13 L-canavanine 23% inhibition at 20 mM Rattus norvegicus
2.6.1.13 L-isoleucine 19% inhibition at 50 mM Rattus norvegicus
2.6.1.13 L-leucine 17% inhibition at 50 mM Rattus norvegicus
2.6.1.13 L-valine 33% inhibition at 50 mM Rattus norvegicus
2.6.1.13 p-chloromercuribenzoate
-
 Rattus norvegicus
2.6.1.13 valerate 38% inhibition at 25 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.13 additional information
-
 additional information Km for ornithine increases below pH 7.5, Km for 2-oxoglutarate decreases below pH 7 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.13 43000
-
 4 * 43000, SDS-PAGE Rattus norvegicus
2.6.1.13 161000
-
 equilibrium sedimentation analysis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.13 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.13
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.13 kidney
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.6.1.13 -15°C, pH 6-8, 0.01 M potassium phosphate buffer, about 20% loss of activity after 4 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Rattus norvegicus DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.13 tetramer 4 * 43000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.13 66
-
 pH 7.5, 15 min, 85% loss of activity, 60 min, 96% loss of activity Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.13 6.8 8 equally stable at pH 6.8 and pH 8.0 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.13 pyridoxal 5'-phosphate 4 mol of pyridoxal phosphate per mol of enzyme Rattus norvegicus