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Literature summary extracted from

  • Yasuda, M.; Tanizawa, K.; Misono, H.; Toyama, S.; Soda, K.
    Properties of crystalline L-ornithine: alpha-ketoglutarate delta-aminotransferase from Bacillus sphaericus (1981), J. Bacteriol., 148, 43-50.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.13
-
 Lysinibacillus sphaericus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.13 3-Methyl-2-benzothiazolone hydrazone hydrochloride fully reversed by addition of pyridoxal phosphate Lysinibacillus sphaericus
2.6.1.13 beta-Lysine 80% inhibition at 10 mM after 20 min Lysinibacillus sphaericus
2.6.1.13 cadaverine fully reversed by addition of pyridoxal phosphate, 57% inhibition at 10 mM after 20 min Lysinibacillus sphaericus
2.6.1.13 carboxylmethoxyamine fully reversed by addition of pyridoxal phosphate Lysinibacillus sphaericus
2.6.1.13 hydroxylamine
-
 Lysinibacillus sphaericus
2.6.1.13 L-arginine fully reversed by addition of pyridoxal phosphate, 28% inhibition at 10 mM after 20 min Lysinibacillus sphaericus
2.6.1.13 L-Cycloserine fully reversed by addition of pyridoxal phosphate Lysinibacillus sphaericus
2.6.1.13 L-leucine fully reversed by addition of pyridoxal phosphate Lysinibacillus sphaericus
2.6.1.13 L-lysine 35% inhibition at 10 mM after 20 min Lysinibacillus sphaericus
2.6.1.13 L-valine fully reversed by addition of pyridoxal phosphate, 35% inhibition at 10 mM after 20 min Lysinibacillus sphaericus
2.6.1.13 phenylhydrazine fully reversed by addition of pyridoxal phosphate Lysinibacillus sphaericus
2.6.1.13 putrescine fully reversed by addition of pyridoxal phosphate, 63% inhibition at 10 mM after 20 min Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.13 41000
-
 2 * 41000, SDS-PAGE Lysinibacillus sphaericus
2.6.1.13 80000 85000 sedimentation equilibrium method, gel filtration Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.13 L-ornithine + 2-oxoglutarate Lysinibacillus sphaericus
-
 DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?
2.6.1.13 L-ornithine + 2-oxoglutarate Lysinibacillus sphaericus IFO3525
-
 DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.13 Erwinia aroidea
-
-
-
2.6.1.13 Lysinibacillus sphaericus
-
 IFO3525
-
2.6.1.13 Lysinibacillus sphaericus IFO3525
-
 IFO3525
-
2.6.1.13 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.13
-
 Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.13 0.55
-
-
 Staphylococcus aureus
2.6.1.13 0.67
-
-
 Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.13 beta-lysine + 2-oxoglutarate weak Lysinibacillus sphaericus glutamate + 3-amino-7-oxooctanoic acid
-
 ?
2.6.1.13 beta-lysine + 2-oxoglutarate weak Lysinibacillus sphaericus IFO3525 glutamate + 3-amino-7-oxooctanoic acid
-
 ?
2.6.1.13 L-lysine + 2-oxoglutarate weak Lysinibacillus sphaericus glutamate + 2-amino-7-oxooctanoic acid
-
 ?
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Staphylococcus aureus DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Lysinibacillus sphaericus DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Erwinia aroidea DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Lysinibacillus sphaericus DELTA1-pyrroline-5-carboxylate + L-glutamate glutamate-gamma semialdehyde spontaneously cyclizes to form DELTA1-pyrroline-5-carboxylate ?
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Lysinibacillus sphaericus IFO3525 DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Lysinibacillus sphaericus IFO3525 DELTA1-pyrroline-5-carboxylate + L-glutamate glutamate-gamma semialdehyde spontaneously cyclizes to form DELTA1-pyrroline-5-carboxylate ?
2.6.1.13 additional information specific for 2-oxoglutarate Lysinibacillus sphaericus ?
-
 ?
2.6.1.13 additional information specific for ornithine Lysinibacillus sphaericus ?
-
 ?
2.6.1.13 additional information specific for 2-oxoglutarate Lysinibacillus sphaericus IFO3525 ?
-
 ?
2.6.1.13 additional information specific for ornithine Lysinibacillus sphaericus IFO3525 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.13 dimer 2 * 41000, SDS-PAGE Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.13 pyridoxal 5'-phosphate activates Lysinibacillus sphaericus
2.6.1.13 pyridoxal 5'-phosphate 1 mol per mol of enzyme Lysinibacillus sphaericus
2.6.1.13 pyridoxal 5'-phosphate a pyridoxal phosphate protein Lysinibacillus sphaericus
2.6.1.13 pyridoxal 5'-phosphate a pyridoxal phosphate protein Erwinia aroidea
2.6.1.13 pyridoxal 5'-phosphate pyridoxal 5'-phosphate is bound through an aldimine linkage to the epsilon-amino group of a lysine residue Lysinibacillus sphaericus