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Literature summary extracted from

  • Eliot, A.C.; Sandmark, J.; Schneider, G.; Kirsch, J.F.
    The dual-specific active site of 7,8-diaminopelargonic acid synthase and the effect of the R391A mutation (2002), Biochemistry, 41, 12582-12589.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.62 wild-type and R391A mutant Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.62
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.62 R391A increased Km for 7,8-diaminopelargonic acid, no significantly altered crystal structure Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.62 7-Keto-8-aminopelargonic acid
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.62 0.002
-
 8-Amino-7-oxononanoate pH 9, 25°C, wild-type enzyme Escherichia coli
2.6.1.62 0.15
-
 S-adenosyl-L-methionine pH 9, 25°C Escherichia coli
2.6.1.62 0.23
-
 8-Amino-7-oxononanoate pH 9, 25°C, R391A murant Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Escherichia coli biotin synthesis S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.62 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate transfer of alpha-amino group from S-adenosyl-L-methionine to KAPA Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate biotin synthesis Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.62 0.0021
-
 8-Amino-7-oxononanoate pH 9, 25°C, R391A mutant Escherichia coli
2.6.1.62 0.013
-
 8-Amino-7-oxononanoate pH 9, 25°C, wild-type enzyme Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.62 2
-
 7-Keto-8-aminopelargonic acid value above 2, pH 9, 25°C, R391A mutant Escherichia coli